Fatty liver is a common metabolic disorder of dairy cows during the transition period. Historically, the diagnosis of fatty liver has involved liver biopsy, biochemical or histological examination of liver specimens, and ultrasonographic imaging of the liver. However, more convenient and noninvasive methods would be beneficial for the diagnosis of fatty liver in dairy cows. The plasma metabolic...

Overmilking is a matter of concern because it may affect teat condition and udder health. The historic background for overmilking is mainly based on the assumption that all milk should be removed from the udder in order to maximise the milk yield. However, breeding for high milk yields has provided cows with a high alveolar capacity, large cistern capacity, and a higher proportion of cisternal ...

Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...

BACKGROUND AND OBJECTIVES Leptospirosis is an important zoonotic disease caused by Leptospira interrogans. Leptospirosis leads to economical losses in dairy farm industry. The objective of this study was to evaluate the pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR. MATERIALS AND METHODS Two hundred samples (100 urine and 100 blood) were collected fr...

Fatty liver is a common metabolic disorder of dairy cows during the transition period. Historically, the diagnosis of fatty liver has involved liver biopsy, biochemical or histological examination of liver specimens, and ultrasonographic imaging of the liver. However, more convenient and noninvasive methods would be beneficial for the diagnosis of fatty liver in dairy cows. The plasma metabolic...

Protection of the mammary gland against mastitis-causing pathogens is mediated by many factors in thegland and blood circulation. The professional phagocytic cells of bovine udder, polymorphonuclearneutrophils (PMN) and macrophages, comprise the first line of defense against invading mastitis pathogens.Most researchers now accept that the PMN is a key factor in the cows’ defense against intrama...

There are approximately 2.5 million dairy cows in California. Emission inventories list dairy cows and their manure as the major source of regional air pollutants, but data on their actual emissions remain sparse, particularly for smog-forming volatile organic compounds (VOCs) and greenhouse gases (GHGs). We report measurements of alcohols, volatile fatty acids, phenols, and methane (CH4) emitt...

In normal use, milking parlor can cause the release of oxytocin through neuroendocrine reflex, but the use of milking parlor sometimes cause stress and lead to a decrease in milk production. In Indonesia at this time, traditional milking by hand is still widely used by dairy farmers. The purpose of this study is to investigate the use of milking parlor to the physiological and behavioral respon...

Blood and milk concentrations of the acute phase protein lipopolysaccharide-binding protein (LBP) were evaluated in cows with naturally occurring mastitis. Blood and milk samples were collected from 101 clinically healthy dairy cows and 17 dairy cows diagnosed with clinical mastitis, and the LBP concentrations of the samples were measured by an ELISA. Concentrations of LBP were greater in the b...

Twinning in Holstein dairy cows has increased over time concurrent with increased milk production. More than 95% of twins in Holsteins arise due to double ovulations resulting in dizygotic twins. It is now clear that low progesterone during growth of a preovulatory follicle increases the incidence of double ovulation. Increased hepatic metabolism of progesterone as a result of the increased fee...