Bacillus represents microbes of high economic, medical and biodefense importance. Bacillus strain identification based on 16S rRNA sequence analyses is invariably limited to species level. Secondly, certain discrepancies exist in the segregation of Bacillus subtilis strains. In the RDP/NCBI databases, out of a total of 2611 individual 16S rDNA sequences belonging to the 175 different species of...

background and aims: the ectodomain of matrix protein of influenza virus is a weak immunogen that is highly conserved among all subtypes of influenza a virus. tandem repeats of these genes along with linker were used to enhance immunogenicity of m2e protein and so it can be served as a universal vaccine in both humans and livestock. materials and methods: in this study, the sequences of extra-d...

in this study, 5,10,15,20-tetrakis(4-nitrophenyl)porphyrin (tnpp) and its zinc porphyrin complex (zntnpp) were synthesized in situ using the microwave method and identified by uv-vis, ft-ir and 1h nmr. the photostability and photodynamic antimicrobial activity (pact) of these compounds were investigated on pseudomonas aeruginosa and bacillus subtilis under visible light irradiation. mic, mbc an...

the present study was undertaken to screen the soil samples collected in iran for the presence of the bacillus subtilis lipase a gene. the bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and  the polymerase chain reaction (pcr). only four colonies were identified as putative b. subtilis strains and all contained the lipase a ge...

bacillus subtilis k40b was isolated from rice rhizospheres and its potential for production of xylanase was evaluated using biochemical methods. the gene for xylanase in b. subtilis k40b was amplified, sequenced and assigned to the ncbi genebank. the gene size was estimated to be 563 bp encoding 413 amino acids. comparison of the xylanase gene with reference sequences showed that the xylanase o...

background: dielectric barrier discharge (dbd), a source of non-thermal plasma, is used in surface decontamination. objective: to study the effect of dbd plasma treatment, we evaluated the effect of plasma exposure time on inactivation of bacillus subtilis. results: applying the dbd plasma to the culture of b. subtilis caused complete sterilization of the surface without any thermal effects. in...

using environmental-friendly methods in order to remove or reduce oil pollutants and their derivations in the environment are developing. in this study, the biodegrading ability of bacillus subtilis .sp which has been separated from oil-polluted soil was examined. it was revealed that it can reduce surface tension of growth medium and produce bio-surfactant at 37 and 20 degrees centigrade. also...