Bacillus Calmette-Guérin (BCG) has been used for vaccination against tuberculosis for nearly a century. Here, we analyze immunity induced by a live tuberculosis vaccine candidate, recombinant BCG ΔureC::hly vaccine (rBCG), with proven preclinical and clinical safety and immunogenicity. We pursue in-depth analysis of the endogenous mycobacteria-specific CD4(+) T-cell population, comparing the mo...

BACKGROUND: The mode of action Bacillus Calmette-Guérin (BCG) in the treatment patients with non-muscle invasive bladder cancer (NMIBC) is incompletely understood, but recent studies support an association between BCG-induced trained immunity circulating monocytes and disease-free survival. OBJECTIVE: We compared epigenetic profiles from NMIBC early disease recurrence those recurrence-free pati...

The aim of this investigation was to improve the storage stability and survival rate of intravesical BCG product, manufactured with an attenuated strain of Mycobacterium bovis (Pasteur strain 1173P2 of BCG) in the presence of sodium glutamate. Formulations with various concentrations of trehalose (a known protectant) were developed as liquid and lyophilized forms. Formulations were evaluated by...

A hundred and five years ago, Albert Calmette and Camille Guérin began a daunting task, which is unmatched even today, that led to the most widely used vaccine in human history. Despite a century of scientific advances, BCG (an acronym for Bacillus Calmette-Guérin) remains the only vaccine for prevention of tuberculosis. Due to the fact that the use of BCG vaccines will continue, either as a st...

The aim of this investigation was to improve the storage stability and survival rate of intravesical BCG product, manufactured with an attenuated strain of Mycobacterium bovis (Pasteur strain 1173P2 of BCG) in the presence of sodium glutamate. Formulations with various concentrations of trehalose (a known protectant) were developed as liquid and lyophilized forms. Formulations were evaluated by...

In the last 10 yrs, work with experimental laboratory models has developed many new vaccine candidates against tuberculosis (TB). They include subunit vaccines, modified bacilli Calmette-Guérin (BCG) and attenuated Mycobacterium tuberculosis. Phase I clinical trials of new TB vaccine candidates have begun for the first time after 80 yrs of use of BCG. Many of these new trials involve recombinan...

Despite the use of Bacillus Calmette-Guérin (BCG) vaccination for many years, infants and young children exposed to adults with infectious forms of tuberculosis (TB) are at high risk of developing complicated TB disease. This risk is much higher among HIV-infected children, and data on BCG protective efficacy in HIV-infected children is lacking. Recent research on BCG safety in HIV-infected inf...

The effect of methylprednisolone sodium succinate treatment on Bacillus Calmette-Guérin (BCG) immunization was examined in alveolar macrophages of New Zealand White rabbits. Intravenous BCG injection caused a marked increase in cell number and in the hexosemonophosphate shunt activity. There was also a selective augmentation in the activity of acid phosphatase and beta-glucuronidase and an incr...

Abstract Before COVID-19 infection caused the global pandemic in 2020, coronavirus diseases were mainly of veterinary interest. This necessitated development protective and therapeutic measures against spread SARS-CoV-2. Foods containing representatives genus Lactobacillus are an integral part daily menu Bulgarian people. Our hypothesis is based on studies examining its potential for competitiv...

In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both empl...