A total of 434 clinical aerobic gram-negative bacillary isolates were tested against LY127935, a new 1-oxa cephalosporin, and compared with other cephalosporins, penicillins, and aminoglycosides by a broth microdilution technique. Cefotaxime (HR756), a new semisynthetic cephalosporin, and LY127935 were more active, and showed lower minimun inhibitory concentrations (rantes, -O.12 to 2.0 ,ug/ml)...

Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-car...

Tubercle bacilli die much more rapidly under anaerobic than under aerobic conditions. Anaerobically, the rate of death is accelerated by increased CO(2) pressure, and when certain metabolites (lactate for example) are present in the medium. Death of the bacilli under certain conditions of anaerobiosis is accompanied by loss of ability to take the acid-fast stain and by progressive cellular disi...

A critical feature of the latency stage is granuloma formation, with the presence of giant cells. This leads to caseous necrosis and the release of bacilli, which can then be transmitted to new patients and infect them. At this stage, the bacilli are referred to as extracellular bacilli, and their condition is anaerobic. Then, is some cases, calcification occurs, while in others, dissemination ...

1st. Bacteria growth: Vitamin C (Redoxon, L-ascorbic acid sodium (PH 6,4 to 6,6]) was added to solid culture medium (PH 7,0) with different pathogenic bacteria like Pneumococcus, influenza bacilli, coliform bacilli, dysentery bacilli, typhus bacilli, diphtheria bacilli, staphylococcus, streptococcus, meningococcus, B. pyocyaneus, B. subtilis, B. prodigiosus, and bacillus pertussis. It was state...

Latent Mycobacterium tuberculosis exhibits metabolic pathways different from normal bacilli. Mycobacteria undergo metabolic shift leading to alteration in expression of vital cell proteins like catalase, urease etc., during nutrient starvation conditions. In this study, we determined the activity of catalase and urease in nutrient starved cultures. It was observed that catalase activity decline...

Dormancy in non-sporulating bacteria is an interesting and underexplored phenomenon with significant medical implications. In particular, latent tuberculosis may result from the maintenance of Mycobacterium tuberculosis bacilli in non-replicating states in infected individuals. Uniquely, growth of M. tuberculosis in aerobic conditions in potassium-deficient media resulted in the generation of b...

The investigations of Rich (1) and his associates have cast profound doubt on the r61e of allergy in immunity to tuberculosis. Their studies intimate that both the fixation of tubercle bacilli at the site of reinfection and the inhibition of their growth may take place in the absence of exaggerated inflammation. Many studies (2) since then have indicated that there is no strict parallel between...

Populations of tubercle bacilli of human origin exposed in vivo to pyrazinamide and a companion drug, vanished from the tissues of the mouse in so far as could be determined by microscopy, culture, or guinea pig subinoculation. The vanishing did not represent a complete elimination of the tubercle bacilli from all the animals. 90 days after the completion of treatment, tubercle bacilli could be...