نتایج جستجو برای: b lactoglobulin

تعداد نتایج: 900693  

Journal: :The Analyst 2016
Lindsay J Morrison Jennifer S Brodbelt

Characterization of all gas-phase charge sites of natively sprayed proteins and peptides is demonstrated using 193 nm UVPD. The high sequence coverage offered by UVPD is exploited for the accurate determination of charge sites in protein systems up to 18 kDa, allowing charge site to be studied as a function of protein conformation and the presence of disulfide bonds. Charging protons are found ...

2016
Joanna I. Loch Piotr Bonarek Magdalena Tworzydło Agnieszka Polit Barbara Hawro Aneta Łach Eryk Ludwin Krzysztof Lewiński

Functional recombinant bovine β-lactoglobulin has been produced by expression in E. coli using an engineered protein gene and purified to homogeneity by applying a new protocol. Mutations L1A/I2S introduced into the protein sequence greatly facilitate in vivo cleavage of the N-terminal methionine, allowing correctly folded and soluble protein suitable for biochemical, biophysical and structural...

Journal: :The Journal of biological chemistry 1992
B Redl P Holzfeind F Lottspeich

The gene encoding human tear prealbumin, a major component of the protein fraction of tear fluid, was cloned from total cDNA of lacrimal gland by polymerase chain reaction using synthetic oligonucleotides derived from N-terminal amino acid sequences of the purified protein. Sequence analysis and a computer-assisted homology search revealed this protein to be a member of the lipocalin superfamil...

Journal: :iranian journal of biotechnology 2009
karim karimi mohammad taghi beigi nassiri khalil mirzadeh amin ashayerizadeh hedayatallah roushanfekr

the objective of this study was to determine the polymorphism of bovine β-lactoglobulin (β-lg) gene and its association with milk production traits in iranian najdi cattle. blood samples were collected from 80 najdi cattle from the najdi cattle breeding station located in shoshtar, khuzestan. polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) techniqus were used...

Journal: :تولیدات دامی 0
محمدرضا محمدآبادی دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان اکرم محمدی کارشناس ارشد، علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل

beta-lactoglobulin (lgb) is one of the proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands and affects the quality and coagulation of the milk. the aim of this study was to determine allele frequencies in lgb gene of native and holstein cattle in kerman province using pcr-rflp method. genetic polymorphism, for 100 dna samples in the locus was determined by di...

Journal: :Genetics 1994
H Bovenhuis J I Weller

Maximum likelihood methodology was used to estimate effects of both a marker gene and a linked quantitative trait locus (QTL) on quantitative traits in a segregating population. Two alleles were assumed for the QTL. In addition to the effects of genotypes at both loci on the mean of the quantitative trait, recombination frequency between the loci, frequency of the QTL alleles and the residual s...

Journal: :Gut 1987
S Husby N Foged A Høst S E Svehag

The uptake of ovalbumin (OA) from egg and beta-lactoglobulin (BLG) from cow's milk into the blood was investigated for seven hours after a test meal in five children with coeliac disease on a gluten free diet and after gluten challenge, and in five children with normal jejunal mucosa. Ovalbumin was detectable by ELISA in three of five coeliac children (maximal concentrations 8-178 ng/ml serum) ...

2008
Pedro M. Reis Thomas W. Raab Jean Y. Chuat Martin E. Leser Reinhard Miller Heribert J. Watzke Krister Holmberg

In the present study, we use a model gastro-intestinal system to study the influence of different food-grade surface-active molecules (Sn-2 monopalmitin, beta-lactoglobulin, or lysophosphatodylcholine) on lipase activity. The interfacial activity of lipase and surfactants are assessed with the pendant drop technique, a commonly used tensiometry instrument. A mathematical model is adopted which ...

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