Bacillus subtilis cell wall-bound protein CWBP33 is encoded by lytE, a gene expressed during the exponential growth phase. Sequence analysis of LytE, a 33-kDa protein, reveals two domains. The N-terminal domain contains a threefold-repeated motif common to several peptidoglycan binding proteins, while the C-terminal domain, probably carrying the catalytic activity, has homology with certain exo...

The Tn551 insertion site of the autolysis-deficient Staphylococcus aureus mutant RUSAL2 was cloned and used to identify the autolysis gene atl in the parent strain, RN450. The open reading frame for atl was 3768 bp in length, encoding a deduced protein of 1256 amino acids and molecular size of 137,381 Da. The atl gene product is a bifunctional protein that has an amidase domain and an endo-beta...

In this human post-mortem autopsy study, 10 organ tissues from 4 cases with different Post-Mortem Intervals (PMIs) were evaluated. Clinical history, agonal state, refrigeration time, and PMI of 10, 12, 16, >24 hours evaluated to determine histology molecular markers the tissue. Results indicated was not predictive RNA stability (RIN values). We observed time after death influenced body temperat...