A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic inc...

We report a pseudo-outbreak of Rhizobium radiobacter infections resulting from contamination by a saline dispenser in the microbiology laboratory. Isolates from clinical specimens had identical antimicrobial susceptibilities and electrophoretic fingerprints. The episode resolved with autoclaving of the dispenser. This demonstrates the importance of timely, thorough investigation of unusual orga...

BACKGROUND The multiple uses of HyFlex(®) controlled memory (CM) rotary instruments (Coltene-Whaledent, Allstetten, Switzerland) have been recommended after sterilization. The purpose of this study was to analyze the surface defects on HyFlex(®) instruments after use and to determine their ability to return to their original shape after autoclaving. MATERIALS AND METHODS Twenty-four new HyFle...

Extracted human teeth are used in many preclinical courses. While there has been no report of disease transmission with extracted teeth, sterilization of teeth used in the teaching laboratory should be a concern. The purpose of this study was to determine the effectiveness of different sterilization/disinfection methods of extracted human teeth using Bacillus stearothermophilus, a bacteria resi...

Scrapie is a transmissible fatal disease that affects the central nervous system of sheep and goats. The diagnosis of scrapie has traditionally relied on histopathologic demonstration of characteristic lesions in brain sections. In recent years, however, increasing attention has been given to the use of immunodiagnostic methods that focus on detection of a specific protein known as the prion pr...

Earlier work showed that the nutritive value of casein was decreased by dry heat and that the addition of lysine restored its nutritive value (1). The lysine was not damaged materially by the heat treatment, since analyses of acid hydrolysates of heated casein with lysine decarboxylase (2) and chemical isolation as the picrate (3) showed no decrease in the lysine content. Eldred and Rodney (2),...

In recent years, there has been increased interest in the use of nanostructures various industries, such as food, textile, pharmaceutical, electronics, and chemical industries. Most these applications require a proper preparation specific nanomaterials. this study, we characterized silver nanoparticles (AgNPs) stabilized with polyvinylpyrrolidone prepared aqueous suspensions using dynamic light...