ATP-binding cassette transporters are powered by ATP, but the mechanism by which these transporters hydrolyze ATP is unclear. In this study, four crystal structures of the full-length wild-type maltose transporter, stabilized by adenosine 5'-(β,γ-imido)triphosphate or ADP in conjunction with phosphate analogs BeF(3)(-), VO(4)(3-), or AIF(4)(-), were determined to 2.2- to 2.4-Å resolution. These...

Vascular senescence is a key component in the initiation of atherosclerosis and its related cardiovascular disorders. C-X-C Motif Chemokine Ligand 12 (CXCL12), CXC chemokine receptor 4 (CXCR4) ligand known to promote atherosclerosis. However, role telomeres CXCL12-mediated senescence-induced remains obscure. This study aimed unravel CXCL12 association with context menopause-induced arterial Apo...

The ATP-binding cassette transporter MDL1 of Saccharomyces cerevisiae has been implicated in mitochondrial quality control, exporting degradation products of misassembled respiratory chain complexes. In the present study, we identified an unusually long leader sequence of 59 amino acids, which targets MDL1 to the inner mitochondrial membrane with its nucleotide-binding domain oriented to the ma...

Vitamin D receptor (VDR) and liver X receptor (LXR) are nuclear receptors, which regulate gene transcription upon binding of their specific ligands. VDR seems to play a role in the regulation of prostate cancer cell proliferation. ATP-binding cassette transporter A1 (ABCA1) is known to be a target gene of LXR and it has been reported to be inhibited by androgen and to be involved in the regulat...

Objective: We previously reported smooth muscle cells (SMCs) represent ?50% of foam in human coronary and ?70% apoE (apolipoprotein E)-deficient mouse aortic atheromas exhibit reduced expression the cholesterol exporter ABCA1 (ATP-binding cassette transporter A1). A major stimulus for is flux out lysosomes, generated by hydrolysis lipoprotein cholesteryl esters LAL (lysosomal acid lipase). In t...

The acidic polysaccharide alginate represents a promising marine biomass for the microbial production of biofuels, although the molecular and structural characteristics of alginate transporters remain to be clarified. In Sphingomonas sp. A1, the ATP-binding cassette transporter AlgM1M2SS is responsible for the import of alginate across the cytoplasmic membrane. Here, we present the substrate-tr...