introduction: mcp (membrane cofactor protein or cd46) is a complement regulatory proteins (crps), which is present in most of body cell membranes (except erythrocytes), and also in most biologycal body fliuds. due to the important role of this protein in the complement system, specially in fertility, the aim of this study was to determine and isolate this protein in human seminal plasma. materi...

Cell membranes display a range of receptors that bind ligands and activate signaling pathways. Signaling is characterized by dramatic changes in membrane molecular topography, including the co-clustering of receptors with signaling molecules and the segregation of other signaling molecules away from receptors. Electron microscopy of immunogold-labeled membranes is a critical technique to genera...

Background & Objective: Diabetic retinopathy (DR) is a common microvascular complication of type 2 diabetes mellitus (T2DM) and the leading cause of vision loss in working-age adults. Vitamin A (retinol) has a role in the mechanism of vision process and retinol binding protein-4 (RBP4), is a carrier of vitamin A, and as an adipokine may be associated with increased risk for insulin resistance a...

Prostate-specific antigen (PSA) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a CM-Sephadex C-50 and by gel filtration on a Sephacryl S-200 column. A single 33-kDa protein band appeared in SDS-PAGE. High pressure liquid chromatography (HPLC) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...

Background: There are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. There are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. Ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

Background: The aim of our study was to determine the association low levels pregnancy associated plasma protein-A (PAPP-A) estimated at 11-13+6weeks gestation with fetal growth restriction (FGR).Methods: A prospective observational a total 203 pregnant women PAPP-A were followed up and outcome data collected childbirth.Results: incidence FGR 7.3%. significant found between MoM (≤0.49) (p=0.000...

Serum concentrations of pregnancy-associated plasma protein A (PAPP-A) were measured in patients with pregnancy-related gynaecologic emergencies including ectopic pregnancy (n = 124) and intrauterine abortion (n = 40). The results were compared with those in normal pregnancy (n = 136) and non-pregnant women (n = 460). In ectopic pregnancy and intrauterine abortion, the PAPP-A levels were lower ...

This enzyme immunoassay procedure for pregnancy-associated plasma protein type A involves a "sandwich"-type system in microtitration plates. One can detect 0.27 mg of the protein per liter of serum, with a between-batch CV of 10.2%, and the antiserum used does not cross react with any of the other placentally derived proteins. A reference interval for the last trimester of pregnancy is presente...

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...