The irritation of cosmetics and their ingredients on lips has mostly been estimated by using human lips. However, human tests are a heavy burden on subjects, making it difficult to evaluate multiple samples. Therefore, it was examined whether the HeLa-MTT assay, which is used as a substitution method for eye irritation test, could be used as a method for evaluating the irritation on lips. Oils ...

The MTT assay, as a screening method, has been widely used to measure the viability and proliferation of cells. However, it should be noted that MTT assay may not accurately reflect the effect of Cistanche tubulosa ethanolic extract on EA.hy926 cells viability. To investigate and identity the components responsible for the contradictory observations of the MTT assay, echinacoside and acteoside,...

to a culture base, their cell-to-cell interactions become very tight, so that they cannot be dispersed perfectly by the convcntional techniques (e.g,, trypsin,iEDTA treatment),i'!) To resolve this problem, we tried to apply a colorimetric MTT assay to primary cultured hepatocytes. The MTT assay is a rapid colorimetric microtiter assay which was originally developed to measure the living cell nu...

The genus Centaurea with an extensive background in Iranian traditional medicine represents more than 70 species in Iran that some of them are endemic to Iran. A variety of secondary metabolites has been isolated from this genus thus far. Sesquiterpene lactones and flavonoids have been reported as main compounds of C.bruguierana. Methanolic extract and different fractions of the whole fruiting ...

Calcium hydroxide had been used as the intracanal dressing in endodontic treatment due to its high alkaline and high antimicrobial capacity. It also be able to dissolve the necrotic tissue, prevent the root resorbtion and regenerate a new hard tissue. The aim of this study is to identify the concentration of calcium hydroxide that has the lowest citotoxicity. There are 5 groups, each group had ...

The MTT antibacterial assay is an important method in natural component discovery. Researchers can use the to quickly and efficiently evaluate activity of compounds. aim this research was find a reduce background colour bacterial cell interference when using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) study effect phytochemicals. This used NZ ‘Hass’ ripe avocado seed ext...