Cell free fetal DNA (cff DNA) is apoptotic that shed from placenta and flows in maternal blood. This can be used as an indicative biomarker to rule out pregnancy associated complications. The objective of this study investigate the correlation concentration cff with intrauterine deaths. RHD gene (Rh blood group, D antigen) was marker for confirmation GAPDH (Glyceraldehyde-3-phosphate dehydrogen...

Cancer is one of the leading causes death in our age. In addition to treatments used cancer, plants are frequently complementary or alternative approaches. Plants genus Satureja world wide for various purposes, primarily as therapies. For this purpose, study, antioxidant potential and phenolic content cuneifolia plant, which consumed a spice tea among public, its cytotoxic apoptosis-inducing ef...

Background: Valproic acid (VPA), a branched short-chain fatty and histone deacetylase (HDAC) inhibitor, has diverse biological activities in human cells, including anti-cancer properties. Objectives: In the present study, we tested cytotoxicity of VPA on proliferation, cell cycle, apoptosis cervical cancer line, HeLa. Methods: HeLa line was cultured Dulbecco’s modified eagle medium (DMEM) effec...

Chronic inflammation plays a key role in the development and progression of several chronic diseases. Inhibiting inflammatory cascade, thereby minimising damage caused by mediators, can be one strategies disease management. In addition, is closely related to apoptosis, cause apoptosis. Lemon peel has been reported have antioxidant anti-inflammatory biological activities. This study aimed invest...

global cerebral ischemia (gci) and reperfusion induced apoptosis that lead to cell injury and death. the bax and bcl-2 are pro-apoptotic and anti-apoptotic genes, respectively. these genes belong to the b-cell lymphoma-2 (bcl-2) family.in this study; we assessed the effect of pentoxifylline drug on bax/bcl2 gene dosage expression changes following   ischemic reperfusion injury in kidney. in thi...

Manuscript submitted 74 AbSTRACT Purpose By expression profiling of 31 apoptosis related genes in acute myeloid leukemia (AML) during the course of the disease we determined whether relapse is caused by emergence of apoptosis resistant subpopulations at diagnosis. Experimental design Samples were obtained from patients diagnosed with AML; 40 at diagnosis, 35 during follow-up and 20 at relapse. ...

We describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell types. A plasmid carrying green fluorescent protein (GFP) is co-transfected with an expression vector encoding the gene of interest. Subsequently cells are stained with propidium iodide and, utilising flow cytometry, transfected, GFP-expressing single c...