One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to e...

The wide occurrence of antimicrobial-resistant (AMR) bacteria in various environments is great concern. Here, we examined the prevalence and antimicrobial susceptibility Enterobacteriaceae isolated from 88 wild arthropods, collected Gifu city, Japan. In total, 168 isolates were obtained 61 arthropods. All susceptible to all agents tested, except colistin (31 isolates) kanamycin (one isolate). a...

Lincomycin, chlortetracycline, and amoxicillin are commonly used antimicrobials for growth promotion and infectious disease prophylaxis in swine production. In this study, we investigated the shifts and resistance development among intestinal microbiota in pregnant sows before and after lincomycin, chlortetracycline, and amoxicillin treatment by using phylogenetic analysis, bacterial enumeratio...

OBJECTIVE To characterize the diarrheal patients with Salmonella typhimurium (S. typhimurium) infections and to set up the first baseline for S. typhimurium pulsed-field gel electrophoresis (PFGE) patterns in Henan province, thus laying a foundation for comprehensive surveillance of Salmonella in human as well as foods. METHODS S. typhimurium isolates recovered from outpatients with diarrhea ...

AIMS To determine the effect of antimicrobial selective pressure on the transmission of antimicrobial resistant and sensitive strains of Salmonella in poultry. METHODS AND RESULTS Eight pens housed 12 broiler chicks each. Two chicks in four of the pens were inoculated with a Salm. Typhimurium strain resistant to 12 antimicrobials (including tetracycline), and two chicks in each of the four ot...

The emergence of multidrug resistance among gram-negative bacilli is complex. Numerous factors need to be considered, including the biological fitness cost of resistance, fitnesscompensatory mutations and frequency and type of antibiotic exposure. A mathematical model evaluating these complex relationships was developed in an individual colonized with strains of pan-susceptible, single-, twoand...

objectives the aim of this study was to determine the 3 most prevalent bacterial pathogens including acinetobacter baumannii, pseudomonas aeruginosa and staphylococcus aureus causing nosocomial infections and their antimicrobial resistant profiles in patients admitted to three hospitals in tehran city, iran. materials and methods in this cross-sectional study, the a. baumannii, p. aeruginosa an...

The aquaculture industry has grown dramatically, and plays an important role in the world's food supply chain. Antimicrobial resistance in bacteria associated with food animals receives much attention, and drug use in aquaculture is also an important issue. There are many differences between aquatic and terrestrial management systems, such as the methods used for administration of drugs. Unique...

Untreatable bacterial infections (“the post-antibiotic era”) are far more likely to emerge in companion animals than in food animals, and have already arrived in the form of the multi-drug resistant (MDR) ESKAPE pathogens, the scourge of hospitals. These highly resistant, and in some cases hospital-adapted pathogens, are Enterococcus faecium, methicillin-resistant Staphylococcus pseudintermediu...

The objective of this study was to estimate the prevalence and the antimicrobial resistance of E. coli that contaminates raw chicken liver as one of the most popular poultry sub-product sold in retail markets. Two hundred and forty samples of fresh raw chicken liver were obtained from national poultry producers in different retail marketing sites and tested for the presence of E. coli. One hund...