The alpha-fetoprotein (afp) receptor (recaf) is an oncofetal antigen found in most types of cancer. Using a competitive radioimmunoassay, we measured the concentration of serum recaf in three sets of samples.Set 1 was blind and consisted of 119 normal subjects, 43 breast cancer patients (stages i and ii), and 20 patients with benign breast conditions. In this set, the assay discriminated normal...

The Phadebact Pneumococcus Test is a coagglutination slide test for the serological identification of Streptococcus pneumoniae. Of 200 alpha-hemolytic streptococcal isolates, coagglutination test results agreed with those of optochin susceptibility and bile solubility in 189 cases, 105 of which were identified as S. pneumoniae by all three methods. The Phadebact test was 100% (113 of 113) sensi...

A counterimmunoelectrophoresis (CIE) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of Bordetella pertussis in 59 patients. A positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87%) of cases. For sera, CIE had a sensitivity of 85% and a specificity of 94%; for urine samples the sen...

BACKGROUND Diagnosis of tuberculosis (TB) remains problematic despite many new advanced diagnostic methods. A reliable and rapid diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnoses of TB. In the present study we describe a prospective evaluation for demonstrating Antigen (Ag) 85 complex in the sera from TB patients. M...

Three susceptible mouse strains, i.e., BALB/c (H-2d), C57BL/6 (H-2b), and major histocompatibility complex-congenic BALB.B10 (H-2b), were infected intravenously with 4 x 10(6) CFU of live Mycobacterium bovis BCG and analyzed 4 weeks later for in vitro spleen cell cytokine secretion in response to purified protein derivative (PPD), BCG culture filtrate (CF), BCG cellular extract, total BCG, the ...

Recombinant phage clones, TB1 and TB2, were selected from a Mycobacterium tuberculosis lambda gt11 DNA expression library by screening with a polyclonal antiserum raised against the antigen 85 complex of Mycobacterium bovis BCG. Analysis of recombinant DNA inserts and expressed fusion proteins showed that two new genes had been isolated. The product of clone TB2 was identified as a member of th...

Problem analysis in designing thematic maps and user interfaces which assist map designers in incorporating their ideas into maps are presented. Problems in designing choropleth maps are analyzed from the map designers point of view. A graphical user interface, called "Histogram Interface" is implemented as a countermeasure for problems in designing choropleth maps. The user interface can assis...

Claudin-3 (CLDN3) and claudin-4 (CLDN4) are the major structural molecules that form tight junctions (TJs) between epithelial cells. We found that knockdown of the expression of either CLDN3 or CLDN4 produced marked changes in the phenotype of ovarian cancer cells, including an increase in resistance to cisplatin (cDDP). The effect of CLND3 and CLDN4 on cDDP cytotoxicity, cDDP cellular accumula...

The text analyzes the contradictions, ambiguities and accommodations underlying the remodelling of Portugal from an imperial metropolis into a European post-colonial nation. It focuses on two interrelated and contradictory facets of national redefinition – namely, the incorporation of Portuguese transnational migrants into the nation and Portugal’s transformation into a receptor of immigrants f...