Semen from six adult male Black Bengal goats (Capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. Each collected samples was divided into two parts: One part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚C) and equilibrated for freezing.In both cases, semen was dilu...

Background: The application of laparoscopic method for intrauterine insemination is limited in sheep industry by the high cost of equipment and low pregnancy rate of frozen-thawed spermatozoa. Both disadvantages could be decreased if chilled ram semen is transported to the farms and inseminated through cervix. Semen extender provides the requirements for sperm and affects its motility, viabilit...

The present work was designed to study the effect of different concentrations of bovine serum albumin (BSA) in extender on post-thaw quality of buffalo spermatozoa. Semen was collected from three buffalo bulls with artificial vagina (42°C) for three weeks (replicates). Qualifying semen ejaculate from each bull was split into five aliquots and diluted in tris-citric egg yolk extender containing ...

Elevating the level of specific omega-3 fatty acids in equine semen by dietary modification can improve motion characteristics of cooled-stored and frozen-thawed sperm. Stallions, which produce semen that has poor tolerance to cooling and freezing, would benefit most from this dietary modification.

This study was carried out to compare the semen characteristics, frozen-thawed sperm viability and testosterone concentration and in vitro fertilization (IVF) and development of in vitro matured pig oocytes between two Yorkshire boars. Semen and blood samples were collected once per week from October to November 2002 from two adult Yorkshire boars at 18 months of age with 170 kg body weight. Sp...

A field trial was conducted on 36 farms in the Netherlands to compare the fertilizing capacity of fresh and frozen-thawed boar spermatozoa. Four-hundred and fifty-one sows were artificially inseminated with semen that had been frozen and thawed according to the Beltsville Method or diluted in Kiev extender and inseminated on the day of collection. Twelve boars of the Dutch Landrace and Dutch La...

The aims of the present study were: (1) to determine the existence of sperm subpopulations with specific motility characteristics in fresh ejaculates from Holstein bulls, (2) to investigate the effects of semen cryopreservation and post-thaw incubation on the distribution of spermatozoa within the different subpopulations, and (3) to evaluate the existence of between-bull variation in the sperm...

The cryoresistance of bucks’ semen collected by transrectal ultrasound-guided massage the accessory sex glands (TUMASG) and electroejaculation (EE) was compared during breeding season (Study 1) or late non-breeding 2). Semen from 10 Gabon bucks with both methods in each then frozen according to a standardized protocol. time required for ejaculation, number electrical pulses applied, sum applied...