SLC26A6 (or putative anion transporter 1, PAT1) is located on the apical membrane of mouse kidney proximal tubule and mediates Cl-/HCO3- exchange in in vitro expression systems. We hypothesized that PAT1 along with a Cl-/HCO3- exchange is present in apical membranes of rat kidney proximal tubules. Northern hybridizations indicated the exclusive expression of SLC26A6 (PAT1 or CFEX) in rat kidney...

Utilizing a pH-stat method, the rates of mucosal and serosal alkalinization were measured separately in the seawater eel intestine. These two rates were dependent on contralateral HCO3" concentration and were inhibited by contralateral application of DIDS, an inhibitor of HCO3~ transport, indicating that the mucosal and serosal alkalinization are due to HCO3~ secretion and absorption, respectiv...

INTRODUCTION The objective of the study is to determine the level of agreement between measured total carbon dioxide (TCO2) and calculated bicarbonate (HCO3-) in our laboratory. MATERIALS AND METHODS TCO2 and HCO3- values of 1820 samples drawn at the same time from the patient were compared. TCO2 from venous samples was measured on Dimension RxL while HCO3- was obtained from arterial blood ga...

The effects of hypercapnia (1% CO2), and the independent effects of changes in extracellular pH (pHe), PCO2 and [HCO3-] on intracellular pH (measured by the DMO method) and lactate metabolism (measured by utilization of 14C-labelled lactate), were examined in rainbow trout hepatocytes in vitro. Simulated uncompensated hypercapnia (high PCO2, low pHe, moderately increased [HCO3-] led to a substa...

One of the major tasks of the renal proximal tubule is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered HCO3- as well as generating new HCO3- for regulating blood pH. This review summarizes the cellular and molecular events that underlie four major processes in HCO3- reabsorption. The first is CO2 entry across the apical membrane, which in large part ...

1. Aedes dorsalis larvae are one of the few organisms capable of inhabiting hypersaline NaHCO3-CO3 lakes. Under laboratory conditions larvae can survive and develop normally in saline media with pH values of 10-5, HCO3~ concentrations of 250 mM, or CO3 ~ concentrations of 100 mM. 2. Despite ingestion of alkaline media at a rate equal to 130% of larval body wt/day, these insects regulated haemol...

Several studies suggest the involvement of Na+ and HCO3- transport in the formation of cerebrospinal fluid. Two Na+-dependent HCO3- transporters were recently localized to the epithelial cells of the rat choroid plexus (NBCn1 and NCBE), and the mRNA for a third protein was also detected (NBCe2) (Praetorius J, Nejsum LN, and Nielsen S. Am J Physiol Cell Physiol 286: C601-C610, 2004). Our goal wa...

The distribution of Na(+)-independent Cl(-)-HCO3- exchange was studied in individual intercalated cells from in vitro perfused rabbit outer CCDs using dual excitation laser scanning confocal microscopy by measuring the pHi response to sequential removal of Cl- from both sides of the tubule. Three patterns of intracellular pH (pHi) response were observed. 39% of intercalated cells had only apica...

Abstract The hydrogeochemical analysis and numerical simulation were conducted to explore the impact of land reclamation on submarine groundwater discharge evolution in seawater intrusion-retreat processes eastern Jiangsu, China. results indicated that origin salt most is saltwater mixing, weathering-dissolution minerals enriches fresh groundwater. path coastal Jiangsu Ca-Na-HCO3-Cl ? Ca-Na-Cl-...