نتایج جستجو برای: and bphg genes cloned into pgreen vector were transformed into ecoli

تعداد نتایج: 17009540  

Esmaeili , Aghil, Ghofrani, Mahdi, Honari, Hossein, Safaei, Sadegh,

Background & Objective: In order to enhance the expression of soluble proteins and facilitate their purification and development of multi-functional polypeptide , chimerical recombinant proteins have been invented . The purpose of this study was to construct ctxB-gfp-stxB gene cassette to measure the uptake and excretion of chimerical antigen in future studies.   Materials & Methods: After prep...

Journal: :iranian journal of biotechnology 2009
mahboobe ghaedi abbas sahebghadam lotfi masoud soleimani mehdi shamsara sare arjmand

in this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (aat) with correct post-translational modifications, a protein production system based on the transduction of cho and cos-7 cells using lentiviral vectors was developed. human aat cdna was cloned into a replication-defective lentiviral vector. the transgene aat-jred chimer was transferr...

Journal: :iranian red crescent medical journal 0
mohammad rabiee-faradonbeh department of microbiology and immunology, faculty of medicine, cellular and molecular research center, shahrekord university of medical sciences, shahrekord, ir iran; department of microbiology, shahrekord branch, islamic azad university, shahrekord,ir iran davood darban sarokhalil department of microbiology and immunology, faculty of medicine, alborz university of medical sciences, karaj, ir iran mohammad mehdi feizabadi department of microbiology, faculty of medicine, tehran university of medical sciences, tehran, ir iran amirhooshang alvandi department of microbiology, faculty of medicine, tehran university of medical sciences, tehran, ir iran hasan momtaz department of microbiology, shahrekord branch, islamic azad university, shahrekord,ir iran neda soleimani department of pathology, al-zahra hospital, isfahan university of medical sciences, isfahan, ir iran

conclusions the results of this study demonstrated that the p450 cyp141 gene was successfully cloned into a pet26b plasmid vector as an expression vector. in this paper, for the first time in iran, this gene was cloned for more purposes, including the expression and purification of the recombinant cytochrome p450 cyp141 protein. results the cloning of p450 cyp141 gene was confirmed by the enzym...

Background & Aims: Salmonella Typhimurium is a negative-gram, non-spore, free capsule, moving bacteria with Trish Perry flagella. Salmonella is the most common cause of food poisoning. The ability to enter and survive in host cells is the condition for pathogenic Salmonella species. Proteins of invasive Salmonella are transferred to the host cells by bacteria. This study was performed for gener...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه اصفهان - دانشکده زبانهای خارجی 1389

this study was conducted to investigate the impact of portfolio assessment as a process-oriented assessment mechanism on iranian efl students’ english writing and its subskills of focus, elaboration, organization, conventions, and vocabulary. out of ninety juniors majoring in english literature and translation at the university of isfahan, sixty one of them who were at the same level of writing...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده علوم پزشکی 1389

introduction: diabetes mellitus is an growing national and international public health concern. the number of people affected by diabetes in world by 2030 will be 69% in developing countries. regular physical activity plays a key role in the management of type 2 diabetes melitus, particularly glycemic control. it has been recommended that peoples with type 2 diabetes participate in moderate-int...

Journal: :علوم گیاهان زراعی ایران 0
سجاد رشیدی منفرد دانشجوی سابق دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج و استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه تربیت مدرس، تهران محمدرضا نقوی استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج بهمن یزدی صمدی استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج هوشنگ علیزاده استادیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج کی یانگ گروه بیوشیمی گیاهی دانشگاه لینیوس سوئد اینلی لوندگرن گروه بیوشیمی گیاهی دانشگاه لینیوس سوئد پیتر برودلیوس

artemisia annua is an annual herb native to asia most probably china produce one of the most important sesquiterpene namely artemisinin which has antimalarial and anticancer activity. three transcription factors belonging to wrky family, two transcription factors belonging to myb family and two enzymes dbr2 and opr3 were isolated from a.  annua leaves and first inserted into pjet1.2 vector then...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه اصفهان - دانشکده زبانهای خارجی 1390

this study was conducted to investigate the impact of podcasts as a learning and teaching tool on iranian efl learners’ motivation for listening as well as on their listening comprehension ability. the study also investigated the learners’ perception towards listening to podcasts and examined whether the learners were likely to accept podcasts. out of fifty-five intermediate learners studying e...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه اصفهان - دانشکده زبانهای خارجی 1388

the purpose of the present study was to investigate the effect of task-based instruction of vocabulary on the receptive and oral productive acquisition of english vocabulary and compare the results with those obtained from the traditional method. the method and procedure applied in this study was as follows: after the implementation of opt, a group of sixty female students were chosen. the stu...

Journal: :international journal of endocrinology and metabolism 0
hossein piri department of biochemistry and nutrition, school of medicine, hamadan university of medical science, ir iran bahram kazemi cellular and molecular biology research center, shahid beheshti university of medical science, ir iran; biotechnology department, faculty of medicine, shahid beheshti university of medical science, ir iran mohsen rezaei department of biochemistry and nutrition, school of medicine, hamadan university of medical science, ir iran mojgan bandehpour cellular and molecular biology research center, shahid beheshti university of medical science, ir iran; biotechnology department, faculty of medicine, shahid beheshti university of medical science, ir iran iraj khodadadi department of biochemistry and nutrition, school of medicine, hamadan university of medical science, ir iran taghi hassanzadeh department of biochemistry and nutrition, school of medicine, hamadan university of medical science, ir iran

background type 1 diabetes mellitus is one of the metabolic diseases that cause insulin-producing pancreatic ß cells be destroyed by immune system self-reactive t cells. recent­ly, new treatment methods have been developed including use of the stem cells, ß islet cells transplantation and gene therapy by viral and non-viral gene constructs. objectives the aim of this project was preparing the n...

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