Purified DNA polymerase from AMV was exposed to thermal inactivation in the presence and absence of various compounds required for maximal activity. A synergistic protective effect was noted when all the components were present. The enzyme showed a distinct preference for primed templates, especially for its native 70S RNA. Heat inactivation studies also suggested that the active sites for RNas...

Sea-level rise is one of the most critical consequences global warming, with potentially vast impacts on coastal environments and societies. changes are spatially temporally heterogeneous multiannual-to-multidecadal timescales. Here, we demonstrate that observed rate winter sea-level in Italian city Venice contains significant multidecadal fluctuations, including interdecadal periods near-zero ...

Alfalfa mosaic virus (AMV) is one of the most important viral diseases alfalfa plant among forage crop, causing significant annual economic losses. The agent also potential importance to other cultivars such as tomatoes, potatoes, and peppers in cases. identification phylogenetic relationships AMV were carried out by reverse-transcription polymerase chain reaction (RT-PCR), following bacterial ...

Abstract We analyze multidecadal temperature fluctuations of the Atlantic Ocean and their influence on Northern Europe, focusing Baltic Sea, without a priori assuming linear relationship this teleconnection. Instead, we use method low-frequency component analysis to identify modes variability in Sea signal relate climate variability. Disentangling seasonal impact reveals that large fraction win...

Atmospheric motion vectors (AMVs) derived with high resolution satellite data are useful for analyzing mesoscale motion such as convective clouds and ageostrophic flow. National Institute of Meteorological Research at Korea Meteorological Administration (KMA/NIMR) has developed a mesoscale AMV algorithm. In the mesoscale AMV algorithm, visible channel data with 1 km resolution from the geostati...

The intrinsic properties of reverse transcriptase in reverse transcription were studied using a synthetic, partial ovalbumin mRNA with a synthetic DNA oligonucleotide annealed to the 3'-end of the RNA as a model substrate. With or without concomitant cDNA synthesis, the RNase H activity of avian myeloblastosis virus (AMV)-reverse transcriptase cleaved the substrate at a site which would leave a...

Monospecific antiserum prepared against the isolated deoxyribonucleic acid (DNA) polymerase of avian myeloblastosis virus (AMV) neutralized the endogenous ribonucleic acid-instructed DNA polymerase activity of detergent-disrupted virus. The viral polymerase was serologically unrelated to the seven major structural polypeptides of AMV. Furthermore, the viral enzyme was distinguished from normal ...

Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial a...

We compared the thermal stabilities of wild-type recombinant avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) with those of mutants of the recombinant enzymes lacking RNase H activity. They differed in resistance to thermal inactivation at elevated temperatures in the presence of an RNA/DNA template-primer. RNase H-minus RTs retained the abil...