The estimation of heparin in blood by a quantitative chemical method has been attempted by Jaques (1939), Jaques, Monkhouse,. and Stewart (1949), and Monkhouse and Jaques (1950), but several difficulties were encountered. The method of Charles and Scott (1933) needs a very large quantity of blood and is not practical for use in man or small laboratory animals. A method has therefore been devise...

The white-rot fungi produce wide range of extracellular enzymes (especially ligninolytic enzymes) and they can degrade complicated and difficult degradable compounds. An immobilization of enzymes causes their stability, reusability and cheapness, so they could be effectively used in biotechnologies and other branches. In this work we focused on the optimization of isolation procedure namely pre...

The goal of the present study was to investigate production of cellulase in low cost medium by thermotolerant yeast. After screening, an efficient yeast isolate having capability of C1 (exo-gluconase) and Cx (endogluconase) production was isolated and designated as strain R-1. Maximum enzyme production was achieved at 50 °C, pH 5.5 in the medium containing bagasse powder 4% (w/v), and ammonium ...

Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentat...

Phosphate solublization activity of Bacillus subtilis in different carbon and nitrogen sources with insoluble form of phosphate sources like tricalcium phosphate and rock phosphate were studied. TCP solubilization with different carbon sources irrespective of incubation period can be related in the following order: glucose, fructose, arabinose, mannose, sucrose, lactose, and sorbitol. The pH dr...

Rat growth hormone was extracted from an acetone powder of transplantable mammosomatotropic pituitary tumors using buffered ammonium sulphate. Active material, precipitated by increasing the ammonium sulphate concentration to 1.5 M, was fractionated first by diethylaminoethyb cellulose chroma tography and then by preparative polyacrylamide gel electro phoresis. The yield of material in the pure...

To understand the molecular interactions between N,N-dimethylformamide with two families of ionic liquids, the thermophysical properties such as densities and ultrasonic sound speeds have been measured over the entire composition range at 25 C under atmospheric pressure. The materials investigated in the present study include two families of ionic liquids, viz., ammonium salts and imidazolium s...

Background: The compositions of Ferrous sulphate, Agarose and Xylenol orange dye (FAX) and Ferrous sulphate, Gelatin and Xylenol orange dye (FGX) in solution of distilled water and sulphuric acid are two tissue-equivalent gel dosimeters. Ionizing radiation causes oxidation of Fe ion to Fe ions which diffuse through the gel matrix and blur the image of absorbed dose over a period of hours after ...

Background: Surface water and groundwater pollution with various forms of nitrogen such as ammonium and nitrate ions is one of the main environmental risks. The major objectives of this study were to evaluate the capacity of natural zeolite (clinoptilolite) to remove NO3- and NH4+ from polluted water under both batch and column conditions. Methods: The laboratory batch and column experiments we...