Column: Cogent Diamond HydrideTM 4μm, 100Å. Catalog No.: 70000-15P-2 Dimensions: 2.1 x150 mm Solvents: A: DI water + 5 mM ammonium acetate B: 90% acetonitrile/10% DI water/10 mM ammonium acetate Gradient: 0.0 min – 80.0% B 5.0 min 5%B 1 .0 min 80%B 6.0 min to 80%B 1.1 min to 5%B Post Tme: 5 min Flow Rate: 0.5 mL/min. Sample Prep: Glyphosate: 168.0067m/z (M H)Figure A: injection #1, RT = 3.365 m...

Seventeen Thermomyces lanuginosus strains, cultivated on raffinose and sucrose, were ranked on the basis of -galactosidase activities. T. lanuginosus CBS 395.62/b strain showed the highest -galactosidase activity on both investigated carbohydrates. Several carbon sources were tested as potential inducers for the -galactosidase synthesis. On melibiose substrate -galactosidase activity was higher...

The present work describes a simple Reverse Phase HPLC method for the determination of Omeprazole and Domperidone from capsule formulations. The determination was carried out on a Gasco, ODS, C-18 (250× 4.5 mm, 5 micron) column using a mobile phase of Acetonitrile: 0.05M ammonium acetate buffer (pH4) in the ratio of (85:15). The flow rate and runtime were 1 ml/min and 10 min, respectively. The ...

In order to determine whether the metabolic state of Geobacteraceae involved in bioremediation of subsurface sediments might be inferred from levels of mRNA for key genes, in situ expression of nifD, a highly conserved gene involved in nitrogen fixation, was investigated. When Geobacter sulfurreducens was grown without a source of fixed nitrogen in chemostats with acetate provided as the limiti...

The binding of polyphosphoinositides (phosphatidylinositol phosphate and phosphatidylinositol bisphosphate) to the antibiotic neomycin is utilized for the purification of these lipids. Neomycin is reductively coupled to reactive glass beads (Glycophase-CPG) and serves as the stationary phase in column chromatography. A total lipid extract is prepared from tissues with chloroform-methanol-KC1 or...

To estimate the influence of the digestive tract luminal ammonia pool on acute toxic effects of cyclophosphamide, the dynamics of blood ammonia, glutamine and urea level, symptoms of toxic action and the survival time have been studied in rats, intraperitoneally treated with cyclophosphamide, at the background of the gavage with non-lethal dose of ammonium acetate (12 mmol/kg, i.e., 0.35 LD50)....

The exponentially growing market of electrochemical energy storage devices requires substitution flammable, volatile, and toxic electrolytes. use Water in salt solutions (WiSE) regarded as green electrolyte might be interest thanks to an association key features such high safety, low cost, wide stability, ionic conductivity. Here, we report comprehensive chemical-physical study circumneutral Wi...

We investigated the interaction of the short peptide nucleic acid (PNA) strand [acccca]-PNA with oligodeoxynucleotides containing one, two, or four tracts of TGGGGT units. Electrospray ionization mass spectrometry allowed exploring the wide variety of complex stoichiometries that were found to coexist in solution. In water, the PNA strand forms short heteroduplexes with the complementary DNA se...