This reaction was found to occur in the case of glycocoll, alanin, or-amidovaleric acid, cz-amido-isovaleric acid, leucin and phenylalanin, and was of especial interest on account of the similarity of the products of oxidation with those obtained through some biochemical oxidative changes. The following experiments were undertaken in order to ascertain whether the reaction could be extended to ...

The goal of this research is to evaluate the performance of two grammatical inference algorithms in the cleavage site prediction problem both in potyviridae poliprotein sequences and in signal peptides. We plan to compare the performance of our algorithms with other approaches available at this time. Cleavage site prediction is a well known problem in bioinformatics, it consists in predict the ...

Accurate and reliable pKa prediction is of significant interest, because it provides direct information of the protonation state of a protein and can be compared to experimental data. In this work pKa shifts of three aminoacids in proteins have been calculated using molecular dynamics free energy simulations with an explicit solvent and implicit Generalized Born solvent model. The direction of ...

Next-generation sequencing (NGS) from SARS-CoV-2-positive swabs collected during the last months of 2022 revealed a large deletion spanning ORF7b and ORF8 (426 nt) in six patients infected with BA.5.1 Omicron variant. This extensive genome loss removed part these two genes, maintaining frame first 22 aminoacids three ORF8. Interestingly, deleted region was flanked by small repeats, which were l...

The spectrum of the protein waste in 36 paediatric patients undergoing abdominal explorationsyv/ho were infused three different concentrations of dextrose solutions without aminoacids is presented. When 10 and 15 per cent dextrose solutions were infused insulin levels increased, free fatty acids decreased and much more protein was wasted. The mean nitrogen losses in groups of 5%, DW, 10% DW and...

In the mouse, ZFP57 contains three classical Cys2His2 zinc finger domains (ZF) and recognizes the methylated TGC(met)CGC target sequence using the first and the second ZFs. In this study, we demonstrate that the human ZFP57 (hZFP57) containing six Cys2His2 ZFs, binds the same methylated sequence through the third and the fourth ZFs, and identify the aminoacids critical for DNA interaction. In a...

As part of an integral structure and function study, we characterized the natural epitope on the EGF recognized by the CB-EGF1 monoclonal antibody, by using peptides displayed on the coat protein of filamentous bacteriophages. Data analyzed demonstrated that the CB-EGF1 monoclonal antibody recognizes a conformational epitope, comprising aminoacids S9, H10, Y13, K28, E40 and R41. The identified ...

The aim of this communication was to describe the detection of the coexistence of HIV-1 variant with dipeptide insertion between codons 69 and 70 of reverse transcriptase. These variants were isolated from a 16-year-old male patient, undergoing treatment in the city of Marilia, SP, Southeastern Brazil. After confirmation of treatment failure, resistance to antiretroviral drugs testing was perfo...

A newly designed quinazoline based compound, 6-pyridin-2-yl-5,6-dihydro-benzo[4,5]imidazo[1,2-c] quinazoline (PDBIQ) has shown the ability for the easy detection of nineteen amino acids on thin-layer chromatography plates as a spray reagent. This new reagent enabled to produce various distinguishable colors with amino acids with different RF values. The detection limits and the binding ability ...