A series of novel 3-/2,3-substituted pyrido[4,3-e][1,2,4]triazino[3,2-c][1,2,4]thiadiazine 6,6-dioxides 4-28 have been synthesized by the reaction of 3-amino-2-(4-thioxo-1,4-dihydropyridin-3-yl-sulfonyl)guanidine with either 2-oxoalkanoic acids and its esters, or phenylglyoxylic hydrates in glacial acetic acid. Some of them exhibited reasonable or moderate anticancer activity toward human cance...

Carbon-13 n.m.r, spectra have been obtained for bovine myelin basic protein at pD 4.4 in D 2 0 and in 6 M guanidine deuterochloride solutions. Chemical-shift differences between resonances from some amino acid residues are interpreted in terms of structured regions in the polypeptide chain of the native protein, whereas the denatured protein displays the spectrum expected for an essentially ran...

A gel chromatographic analytical procedure using Bio-Gel A-OSm in 6 M guanidine HCl-0.1 M Z-mercaptoethanol has been developed and standardized. The procedure permits the molecular weight of peptides in the size range of 2 to 146 amino acids in length to be established from the distribution coefficient of the peptide on the gel column. This procedure has been applied to an analysis of the size ...

Formaldehyde fixation of simian virus 40 (SV40)-infected CV-1 cells at appropriate times after infection permits us to isolate crosslinked complexes of SV40 minichromosomes during the time of DNA replication and during packaging with viral proteins. Such crosslinked complexes can be separated on the basis of density on CsCl/guanidine . HCl density gradients. During the course of these studies w...

Purified threonine deaminase of Salmonella typhimurium was found to be monodisperse and to have a molecular weight of 194,000. The protein, in 6 M guanidine HCI and 0.1 M P-mercaptoethanol, dissociates into polypeptide chains of molecular weight 48,500. Tryptic peptide analysis implies that the four component chains of the native enzyme are of identical amino acid sequence. L-Isoleucine and L-v...

Purified threonine deaminase of Salmonella typhimurium was found to be monodisperse and to have a molecular weight of 194,000. The protein, in 6 M guanidine HCI and 0.1 M P-mercaptoethanol, dissociates into polypeptide chains of molecular weight 48,500. Tryptic peptide analysis implies that the four component chains of the native enzyme are of identical amino acid sequence. L-Isoleucine and L-v...

Applications The Eimer and Amend Photoelectric Colorimeter can be used wherever "visual" colorimetric methods are employed. Published determinations with this type of instrument include the following: Fluorides, HCN (turbidity), Blood sugar, Urine sugar, Blood creatinine, Cholesterol, Hemoglobin, Indican, Bilirubin, Nitrogen, Blood protein, Urine protein, Spinal fluid protein, Urea, Creatine, G...

12-Carboxamido- and 12-carboxyl-11-spirostenes were synthesized from the corresponding 12-iodo-11-ene derivative in palladium-catalyzed carbonylation reactions under mild reaction conditions. The synthesis of the iodo-alkene substrate is based on the transformation of the 12-keto derivative (hecogenin) to hydrazone, which was treated with iodine in the presence of a base (1,1,3,3-tetramethyl gu...