Extensive work with human hemoglobins indicates that the typical variant hemoglobin differs from normal hemoglobin by a single amino acid substitution. MAutational studies with E. coli tryptophan synthetase have shown that variants of this protein also owe their origin to single replacement of an amino acid. Using biochemical and genetic recombination techniques, it has been shown that the site...

D-amino acids are valuable building blocks for the synthesis of biologically active compounds and pharmaceuticals. The asymmetric chiral amino from prochiral ketones using stereoselective enzymes is a well-known but far exhausted approach large-scale production. Herein, we investigated pyridoxal-5′-phosphate-dependent acid transaminase Haliscomenobacter hydrossis as potential biocatalyst enzyma...

A trial was conducted to biochemically explain the decreased lipid deposition and increased protein accretion observed in pigs fed carnitine. Our hypothesis was that an increase in the ratio of acetyl CoA:CoA-SH produced by stimulation of fatty acid oxidation by supplemental L-carnitine may decrease branched-chain alpha-keto acid dehydrogenase activity and increase pyruvate carboxylase activity...

The peptides which result from treatment of the S-[14C]carboxymethyl derivative of dogfish M4 lactate dehydrogenase (EC 1.1.1.27) with trypsin have been isolated and their sequences have been elucidated. Each identical subunit has a molecular weight of 36,000 and on the basis of the amino acid composition 40 unique tryptic peptides are anticipated. Thirty-seven of these peptides have been isola...

Micrococcus luteus naturally produces alkenes, unsaturated aliphatic hydrocarbons, and represents a promising host to produce hydrocarbons as constituents of biofuels and lubricants. In this work, we identify the genes for key enzymes of the branched-chain amino acid catabolism in M. luteus, whose first metabolic steps lead also to the formation of primer molecules for branched-chain fatty acid...

a study was conducted to evaluate efficacyof wheat based vs. corn based diets on growth performance, carcass traits,blood parameters, jejunum morphological development, immunity, cecal microflora and excreta moisture in broiler chicks fed according to digestible essential amino acid profile. one hundred forty day old ross 308 male broiler chicks randomly distributed into ten separated floor pen...

brachystegia eurycoma is predominantly grown in the eastern region of nigeria. its seed is used in food majorly as a soup condiment, flavouring agent and soup thickener. the proximate, mineral and amino acid compositions were determined on raw and processed seeds (boiled, fermented and roasted) using standard analytical techniques. the processing methods showed deviations in nutrients from the ...