The strategy developed for sex determination in skeletal remains is to amplify the highly degraded DNA, by use of primers that span short DNA fragments. To determine sex of unembalmed human cadaveric skeletal fragments from Sokoto, North-western Nigeria, using Polymerase Chain Reaction (PCR). A single blind study of Polymerase Chain Reaction (PCR)-based sex determination using amelogenin gene a...

sequencing of a part of the amelogenin gene. This gene is lecated on the X and Y chromosomes, and there are 54 nucleotide de]etions on the Y chromosome-specific gene. Forty-seven (26 ma]e and 21 female) DNA samples and 23 (13 ma}e and 1O female) DNA samples, respectively extracted from white blood cells and hairs ofJapanese black bears were analyzed, The primers SE47 and SE48 from this X-Y homo...

Amelogenin is a major protein of the developing enamel matrix. There are two amelogenin genes (AMELX and AMELY) located on the X and Y chromosomes, respectively, in dogs. In the present study, we characterized full-length cDNAs and alternative splicing patterns of the AMEL genes in the tooth tissue of a dog by 5'- and 3'-rapid amplification of cDNA ends and AMEL-specific RT-PCR. Sequence analys...

We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on ana...

There is evidence for a genetic component in caries susceptibility, but the disease is greatly influenced by environmental factors, which are extremely difficult to control in humans. For the present study, we used DNA samples collected from 110 unrelated, non-cleft individuals older than 12 years of age from Tiquisate, Guatemala: a population with similar cultural, dietary and hygiene habits, ...

Mutations in human and in mouse orthologous genes Amelx and Enam result in a diverse range of enamel defects. In this study we aimed to investigate the phenotype-genotype correlation between the mutants and the wild-type controls in mouse models of amelogenesis imperfecta using novel measurement approaches. Ten hemi-mandibles and incisors were dissected from each group of Amelx(WT), Amelx(X/Y64...

Inherited diseases caused by genetic mutations can arise due to loss of protein function. Alternatively, mutated proteins may mis-fold, impairing endoplasmic reticulum (ER) trafficking, causing ER stress and triggering the unfolded protein response (UPR). The UPR attempts to restore proteostasis but if unsuccessful drives affected cells towards apoptosis. Previously, we reported that in mice, t...

Amelogenesis imperfecta (AI) is a group of inherited conditions featuring isolated enamel malformations. About 5% of AI cases show an X-linked pattern of inheritance, which are caused by mutations in AMELX. In humans there are two, non-allelic amelogenin genes: AMELX (Xp22.3) and AMELY (Yp11.2). About 90% of amelogenin expression is from AMELX, which is nested within intron 1 of the gene encodi...

Submitted for the MAR11 Meeting of The American Physical Society Brownian dynamics simulations of amelogenin microribbons formation1 WEI LI, ANTHONY PEREZ LOPEZ, YA LIU, Lehigh University, AMIT CHAKRABARTI, Kansas State University, JAMES GUNTON, Lehigh University — Recent advances in chemical particle synthesis have emphasized the fundamental role of surface colloidal heterogeneities and their ...

REFERENCE: LaFountain M, Schwartz M, Cormier J, Buel E. Forensic samples offer a challenge to nearly any analytical Validation of capillary electrophoresis for analysis of the X-Y method. Sample age or origin may be undetermined and available homologous amelogenin gene. J Forensic Sci 1998;43(6): sample volume small. Material submitted for testing may be depos1188–1194. ited on any imaginable s...