The ubiquitous SAR11 and Prochlorococcus bacteria manage to maintain a sufficient supply of phosphate in phosphate-poor surface waters of the North Atlantic subtropical gyre. Furthermore, it seems that their phosphate uptake may counter-intuitively be lower in more productive tropical waters, as if their cellular demand for phosphate decreases there. By flow sorting (33)P-phosphate-pulsed (32)P...

Availability of phosphate for phytoplankton and bacteria and of glucose for bacteria at different pCO2 levels were studied in a mesocosm experiment (PeECE III). Using nutrient-depleted SW Norwegian fjord waters, three different levels of pCO2 (350μatm: 1×CO2; 700μatm: 2×CO2; 1050μatm: 3×CO2) were set up, and nitrate and phosphate were added at the start of the experiment in order to induce a ph...

Since adenosine triphosphate (ATP)l transfers its labile phosphate to glucose, for every mole of triose phosphate oxidized 1 mole of inorganic phosphate is taken up by the fermenting system. Besides this uptake, an internal cycle exists for the second phosphate group, which is already present in the triose phosphate and after oxidation appears finally in the phosphopyruvic acid. This phosphate ...

Uracil-DNA glycosidase, an enzyme that catalyzes the release of free uracil from uracil-containing DNA, has been purified ll,OOO-fold from Escherichia coli cell extracts. The enzyme preparation was essentially homogenous, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The native enzyme is a small monomeric protein of a molecular weight of 24,500 + 1,000. The enzyme e...

Since adenosine triphosphate (ATP)l transfers its labile phosphate to glucose, for every mole of triose phosphate oxidized 1 mole of inorganic phosphate is taken up by the fermenting system. Besides this uptake, an internal cycle exists for the second phosphate group, which is already present in the triose phosphate and after oxidation appears finally in the phosphopyruvic acid. This phosphate ...

Lipopolysaccharides (LPS, O-antigens) of 12 strains of the photosynthetic bacterium Rhodopseudomonas gelatinosa were obtained by the phenol/chloroform/petroleum ether method, recommended for extracting lipophilic glycolipids of enterobacterial R-mutants. All R. gelatinosa LPS have essentially the same chemical composition. Similar to LPS of Salmonella R-mutants of chemotypes Rd1 and Rd2, the so...

Rinderpest virus (RPV) large (L) protein is an integral part of the ribonucleoprotein (RNP) complex of the virus that is responsible for transcription and replication of the genome. Previously, we have shown that recombinant L protein coexpressed along with P protein (as the L-P complex) catalyses the synthesis of all viral mRNAs in vitro and the abundance of mRNAs follows a gradient of polarit...