Evidence is presented that there is a considerable accumulation of inactive fructose-1,6-diphosphate aldolase (EC 4.1.2.7) in the liver of senescent mice. Liver aldolase was purified from 3-month-old mice and used to immunize rabbits. It was demonstrated with the monospecific antibody thus produced that the liver aldolase of young adult (3 month) and aged (31 month) mice are antigenically ident...

The uptake and degradation of radiolabelled rabbit muscle fructose-bisphosphate aldolase (EC 4.1.2.13) was studied in HeLa cells microinjected by the erythrocyte ghost fusion system. Labelled aldolase was progressively modified by treatment with GSSG or N-ethylmaleimide (NEM) before microinjection to determine whether these agents, which inactivate and destabilize the enzyme in vitro, affect th...

Xanthobacter flavus, a gram-negative facultatively autotrophic bacterium, employs the Calvin cycle for the fixation of carbon dioxide. Cells grown under autotrophic growth conditions possess an Fe(2+)-dependent fructosebisphosphate (FBP) aldolase (class II) in addition to a class I FBP aldolase. By nucleotide sequencing and heterologous expression in Escherichia coli, genes encoding transketola...

The apicomplexan parasite Toxoplasma gondii must invade host cells to continue its lifecycle. It invades different cell types using an actomyosin motor that is connected to extracellular adhesins via the bridging protein fructose-1,6-bisphosphate aldolase. During invasion, aldolase serves in the role of a structural bridging protein, as opposed to its normal enzymatic role in the glycolysis pat...

Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by deoxyribose and coordinately regulated with deoxyribose kinase. The second fo...

A method is described for the detection of 2-keto-4-hydroxyglutarate aldolase activity after electrophoresis of the enzyme on polyacrylamide gels. When gels are incubated with substrate (2-keto-4-hydroxyglutarate), activity is seen as a yellow-colored band due to interaction’ of the product (glyoxylate) with ortho-aminobenzaldehyde and glycine. Positive results have been obtained using either c...

OH OH Two main classes of aldolases are found in Nature. Type I aldolases activate the nucleophilic partner by forming an imine as an intermediate; this type of enzyme is found in animals and higher plants. Type II aldolases occur in microorganisms and they require R 2 to be a hydroxy group. These enzymes feature a Zn 2+ ion that promotes the reaction by forming a chelate with both the carbonyl...

BACKGROUND Mutations in the 4-hydroxy-2-oxoglutarate aldolase (HOGA1) gene have been recently identified in patients with atypical primary hyperoxaluria (PH). However, it was not clearly established whether these mutations caused disease via loss of function or activation of the gene product. METHODS Whole-gene sequencing of HOGA1 was conducted in 28 unrelated patients with a high clinical su...

In the original study of 2-keto-3-deoxy-6-phosphogluconic aldolase (I), and in subsequent experiments, the net synthesis of 2-keto-3-deoxy-6-phosphogluconate from the cleavage products could not be demonstrated. The present experiments were undertaken to clarify this point and to test the conformity of the reaction catalyzed by 2-keto-3-deoxy-6-phosphogluconic aldolase to the general characteri...

No. st-j476 Rabbit Muscle Aldolase catalytic mechanism: Trapping of covalent intermediates M. St-Jean, J. Sygusch (Université de Montréal) Beamline(s): X8C Introduction: Rabbit muscle aldolase (EC 4.1.2.13) is a class I fructose-1,6-bisphosphate aldolase that utilizes covalent enzymatic intermediates in its catalytic mechanism [1]. Although the chemical composition of these covalent intermediat...