We report a method to enrich cysteinyl adducts of human serum albumin (HSA), representing biomarkers of exposure to systemic electrophiles. Because the major site of HSA adduction is the single free sulfhydryl group at Cys34, we used thiol-affinity resins to remove mercaptalbumin (i.e., unadducted HSA) from the cysteinyl adducts. Electrospray ionization mass spectrometry was used to detect merc...

We used cysteinyl adducts of serum albumin (Alb) to investigate the production of two reactive benzene metabolites, namely, benzene oxide (BO) and 1,4-benzoquinone (1,4-BQ) in workers exposed to benzene. Adducts were measured in 160 benzene-exposed workers who did not use respiratory protection (based upon individual geometric mean benzene exposure levels: median=5.27 ppm, interquartile range=2...

The formation constants and Gibbs free energy for the interaction of RnSnClm (n = m = 2, R = Me, Ph, n-Bu or n = 4, m = 0, R = Ph) compounds as acceptors, with ML complexes (H2L = N,N'-bis(salicylidene)-2-aminobenzylamine (salabza-H2) and its derivatives, M = Mn2+ or Zn2+), as donors in N,N'-dimethylformamide (DMF) as solvent were studied. These parameters were measured by using UV-vis spectrop...

Exposure to polycyclic aromatic hydrocarbons (PAHs) in foundry workers has been evaluated by determination of benzo(a)pyrene-serum albumin adducts and urinary 1-hydroxypyrene. Benzo(a)pyrene binding to albumin and 1-hydroxypyrene were quantitatively measured by enzyme linked immunosorbent assay (ELISA) and reverse phase high performance liquid chromatography (HPLC), respectively. 70 male foundr...

primary mgcl2.3.3etoh adduct (pct1) was prepared by melt quenching method and then submitted into a programmed thermal dealcoholation project using a fluidized bed reactor. during thermal dealcoholation program, different mgcl2.netoh support samples with n= 3.0, 2.7, 2.4, and 2.1 were selected and named as pct2 to pct5, respectively. structural analysis of the support samples showed a significa...

Two biomarkers of exposure to cigarette smoke, 4-aminobiphenyl-hemoglobin (Hb) adducts and aromatic DNA adducts in lymphocytes, were determined from a population of 55 smokers and 4 nonsmokers. The levels of these adducts were related to daily cigarette consumption and also to (calculated) tar and nicotine intake. The Hb adduct levels seemed to correspond best to the number of cigarettes (cig) ...

A major focus of our research is the characterization and quantitation of carcinogen-protein adducts. This was done in the past primarily by electron-capture NICI-GC-MS of derivatized adduct moieties following their hydrolytic release from the proteins. The actual analyses were thus carried out on small molecules identical to or structurally similar to the original carcinogen. Most experiments,...

We have found that copper(II) compounds containing a peptide group in the chelate exhibit high activity for modification or degradation of albumin in the presence of hydrogen peroxide, whereas no activity was detected for the copper(II) compounds without an amide-group. It is suggested that presence of the amide-group in the ligand may play an important role in the formation of a peroxide adduc...