Direct measurements of aggregation forces in piconewton range between two red blood cells in pair rouleau are performed under physiological conditions using double trap optical tweezers. Aggregation and disaggregation properties of healthy and pathologic (system lupus erythematosis) blood samples are analyzed. Strong difference in aggregation speed and behavior is revealed using the offered met...

We computationally investigated the molecular aggregation effects on excited state deactivation processes by considering both direct vibrational relaxation and S0/S1 surface crossing, that is, minimum energy conical intersection (MECI). Taking classical AIEgens bis(piperidyl)anthracenes (BPAs) isomers substituted silole derivatives as examples, we show deformation of MECI always occurs at atom ...

protein aggregation is a problem in biotechnology. high temperature is one of the most important reasons to enhance enzyme inactivation and aggregation in industrial systems. this work focuses on the effect of tio2 and sio2 nanoparticles on refolding and reactivation of lysozyme. in the presence of tio2 and sio2 nanoparticles, after enzyme heat treatment at 98◦c for 30 min, not only aggregates ...

objective: to study the mechanisms involved in amyloid formation processes, we made a mammalian cell culture model of amylin aggregation and characterized its properties. materials and methods: amyloid fibrils were extracted from cho cells and binding affinity to thioflavin t and congo red was investigated. then the apple-green birefringence of extracted fibrils was detected under polarized li...

This paper describes computer modelling studies using classical Molecular Dynamics techniques and their derivative methods such as umbrella sampling and bias-exchange metadynamics to study protein behavior in various environments causing folding, unfolding and aggregation of proteins. We present applications related to two important proteins – insulin and apolipoprotein C-II (ApoC-II). Key-Word...