Preparation of an agarose derivative (MPE-agarose) containing a maleimido group which is attached to agarose via a cleavable phenyl ester linkage is described. MPE-agarose was shown to react with the thiol groups in glutathione, bovine serum albumin, bovine hemoglobin, and yeast and rabbit muscle glyceraldehyde 3-phosphate dehydrogenase. Treatment of the resulting agarose-linked compounds for 1...

The effect of ionic strength of agarose solution and quenching temperature of the emulsion on the structure and mechanical strength of agarose-based chromatographic adsorbents was investigated. Solutions of agarose containing different amounts of NaCl were emulsified at elevated temperature in mineral oil using a high-shear mixer. The hot emulsion was quenched at different temperatures leading ...

The study of how cell behavior is controlled by the biophysical properties of the extracellular matrix (ECM) is limited in part by the lack of three-dimensional (3D) scaffolds that combine the biofunctionality of native ECM proteins with the tunability of synthetic materials. Here, we introduce a biomaterial platform in which the biophysical properties of collagen I are progressively altered by...

Fatty acids immobilized on agarose have been employed in the isolation and study of serum albumin by affinity chromatography. Various oleyland palmityl-aminoalkylamino-agarose preparations bound about 10 mg of albumin per ml of agarose; other proteins were retained in smaller amounts and with lesser affinity. Fatty acid-agarose columns which had been exposed to human serum and then washed yield...

Mechanical forces are obviously important in the assembly of three-dimensional multicellular structures, but their detailed role is often unclear. We have used growing microcolonies of the bacterium Escherichia coli to investigate the role of mechanical forces in the transition from two-dimensional growth (on the interface between a hard surface and a soft agarose pad) to three-dimensional grow...

To establish a typical tumor model of ovarian cancer which may be more representative and reliable than traditional monolayer culture and pellet, agarose was used as cell vehicle to engineering tumor. Selection of agarose is based on its successful application in tissue engineering with both amenable mechanical and biological properties. In this study, ovarian cancer cell line SKOV3 was encapsu...

Fatty acids immobilized on agarose have been employed in the isolation and study of serum albumin by affinity chromatography. Various oleyland palmityl-aminoalkylamino-agarose preparations bound about 10 mg of albumin per ml of agarose; other proteins were retained in smaller amounts and with lesser affinity. Fatty acid-agarose columns which had been exposed to human serum and then washed yield...

The present studies were undertaken to evaluate the in vitro gel stability of the hydrogels alginate and agarose. Gel strength (of alginate and agarose) and protein diffusion (of alginate only) were shown to correlate with gel stability and to be useful techniques to monitor gel stability over time. The gel strengths of alginate and agarose were followed for a 90-day period using gel strength a...

An agarose overlay yielded mengovirus plaques earlier and in greater size and number than overlays of chemically undefined agars with or without enhancers. Marked variability in plaque-forming efficacy of commercial agarose preparations was noted.