The laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) gene from Neurospora crassa was cloned and part of its nucleotide sequence corresponding to the carboxyl-terminal region of the protein has been determined. The gene was cloned by cDNA synthesis with a laccase-specific synthetic deoxyundecanucleotide as primer and poly(A) RNA isolated from cycloheximide-treated N. crassa cultures as t...

Since Phe-tRNA synthetase of Neurospora crassa reacts with tRNAV”’ of Escherichia coli to produce Phe-tRNAV&‘, the parameters that effect the reverse reaction were examined. Similarly, the interaction of Val-tRNA synthetase (E. coti) with Phe-tRNAVal and Val-tRNAVal (E. coli) was studied. Phe-tRNA synthetase (N. crassa) can catalyze the deacylation of both Phe-tRNAVa 1 (E. coli) and Val-tRNAV”’...

Fungal hyphae and plant pollen tubes are among the most highly polarized cells known and pose extraordinary requirements on their cell polarity machinery. Cellular morphogenesis is driven through the phospholipid-dependent organization at the apical plasma membrane. We characterized the contribution of phosphoinositides (PIs) in hyphal growth of the filamentous ascomycete Neurospora crassa. MSS...

most common tumors of the spleen are hematologic and lymphoid malignancies. non-lymphoid and non-hematologic tumors of the spleen are very rare, the most common of which are vascular tumors. this group of tumors in the spleen is composed of heterogeneous tumors such as hemangioma, angioendothelioma (ae), littoral cell angioma, and angiosarcoma. there are several histologic forms of ae such as e...

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Environmental pH induces a stress response triggering a signaling pathway whose components have been identified and characterized in several fungi. Neurospora crassa shares all six components of the Aspergillus nidulans pH signaling pathway, and we investigate here their regulation during an alkaline pH stress response. We show that the N. crassa pal mutant strains, with the exception of Δpal-9...