Reverse-phase high-performance liquid chromatography (HPLC) resolution and recovery of cytochrome P-450 and bovine rhodopsin, both integral membrane proteins, and large peptides derived from P-450 LM2 were enhanced by utilizing ternary solvents. Surprisingly, most test materials eluted later in the gradient when using mixtures of acetonitrile and propanol in the mobile phase compared to using e...

The synthesis and stability of two bis(iminophosphoryl) substituted lithium chloride carbenoids with different N-substituents (tolyl and adamantyl) were examined. Their preparation was accomplished via the mild oxidation of the corresponding dilithio bis(iminophosphoryl) methandiides, BIPM(Tol) and BIPM(Ad), which are easily accessible by direct double deprotonation. In the case of the adamanty...

Recently, we discovered that the delocalization of nitrogen lone-pair electrons (NLPEs) in five-membered heterocycles created a second σ-aromaticity addition to prototypical π-aromaticity. Such dual-aromatic compounds, such as pentazole anion, were proved have distinct chemistry comparison traditional π-aromatics, benzene, and surprisingly unstable, susceptible electrophilic attack, relatively ...

The object of this investigation has been to study (a) the conditions necessary for obtaining precipitation of vitamin C with basic lead acetate, (b) the use of n-butyl alcohol as a solvent, and (c) the stability of purified preparations stored in the laboratory. The general method employed for concentrating and assaying the antiscorbutic factor from lemons’ was the same as that previously repo...

CONTEXT Quercetin (QR) is a natural strong antioxidant of the plant origins. It is used for treating conditions of heart and blood vessels including "hardening of arteries" (atherosclerosis), high cholesterol, heart disease, circulation problems, preventing cancer, for treating chronic infections of the prostate, etc. AIMS The aim of this work was to development and study of an acid-free tech...

The current examination dealt with the development of a methodology to produce quercetin, multifunctional bio-flavonoid, by designing task-specific, highly acidic deep eutectic solvents, based on glycerol and natural organic acids. purpose was achieve simultaneous hydrolysis spiraeoside, major quercetin glycoconjugate occurring in onion solid wastes, extraction. After an initial performance scr...

Immunodetection of proteins on blot membranes, Wrst developed by Towbin et al. [1], is the method of choice for identifying speciWc proteins after electrophoresis. Due to its high resolution, immunoprobing of blots from twodimensional polyacrylamide gels has been used extensively as a means of localizing speciWc proteins among hundred or even thousands of separated proteins [2]. Three major typ...

This contribution explores the effect of the bridging diamine upon the aggregation properties of a Zn(II) Schiff-base complex, , both in the solid state and in solution. The X-ray structure of , resulting from the harvest of good quality crystals using chloroform and diethyl ether as solvents, shows the presence of a densely packed dimer in the solid state which pentacoordinates two Zn atoms in...