A nucleic acid amplification technique termed as netlike rolling circle amplification is proposed by introducing a nicking enzyme into the existing hyperbranched rolling circle amplification system. Surprisingly dense and uniform network morphology is observed; and cubic amplification is achieved for the sensitive detection of a sequence from HIV.

Surface plasmon resonance imaging technique for nucleic acid detection M.G. Manera a,b,∗, J. Spadavecchia a, A. Leone a, F. Quaranta a, R. Rella a, D. Dell’atti c, M. Minunni c, M. Mascini c, P. Siciliano a a IMM-CNR Lecce via per Arnesano, c/o Campus Scientifico, 73100 Lecce, Italy b ISUFI-Università degli studi di Lecce, viale Gallipoli, 73100 Lecce, Italy c Dipartimento di Chimica, Universit...

BACKGROUND Polyglycolic acid mesh (PAM) reinforcement of colonic anastomoses were evaluated. METHODS Twenty female albino rabbits were divided into two groups. Each rabbit underwent segmental colonic resection with single-layer anastomosis. In one group of rabbits, PAM of length equal to the circumference of the anastomosis was applied. Rabbits were sacrificed on postoperative day 10 and peri...

Objective(s) The liver has major role in the organism homeostasis, interactions with other systems, synthesis and metabolism of bile production, drug detoxification and hormone inactivation. Cholestasis can be defined as an impairment of the bile flow which can lead to hepatocytes necrosis and finally cirrhosis. Some studies reported a gastric acid secretion reduction in cirrhotic subjects, wh...

the arithmetic mean roughness, ra, values were determined by scanning electron microscopic technique of different core models (furazolidone, nitrofurantoin and acetyl salicylic acid), coated by hpmc film forming solution containing 10 wt% of various molecular weight pegs. the results showed that both plasticizer molecular weight and core surface free energy could have a significant influence on...

This paper concerns with the behavior of an electrodeionization (EDI) system for concentration of citric acid from fermentation broth. Commercially cation-exchange membrane (MC-3470) and anion-exchange membrane (MA-3475) were used as ionic selective barriers of the EDI stack. The diluted compartments of the EDI stack were filled with mixed ion-exchange resins (purolite strong acid cation-exchan...

Background: Meat products may be unacceptably tough because of the high connective tissue content of meats used in their manufacture. OBJECTIVE: The aim of this study was using meat acid marination method on some physicochemical and sensory characteristics of beef burgers. METHODS: Meat was marinated in three concentrations (0.5%, 1% and 1.5%) of lactic and citric acid solutions (1:4 w/v) at 4 ...

introduction the concentration of substances, including urea, creatinine, and uric acid, can be used as an index to measure toxic uremic solutes in the blood during dialysis and interdialytic intervals. the on-line monitoring of toxin concentration allows for the clearance measurement of some low-molecular-weight solutes at any time during hemodialysis.the aim of this study was to determine the...

Many factors may influence the efficiency of somatic embryogenesis. This capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. The effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mM) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

pistachio oil has important nutritional and therapeutic properties because of high concentration of essential fatty acids. the extraction method used to obtain natural compounds from raw matter is critical for product quality and especially protection of nutrition value of them. the study was conducted to compare fatty acid composition of pistachio oil extracted by two conventional procedures: ...