Background and Objectives: Sputum smear staining for acid-fast bacilli is initial approach to the diagnosis of pulmonary tuberculosis (PTB) but more than 50% of cases are sputum smear-negative. This study was aimed to investigate the diagnostic value of fiberoptic bronchoscopy (FOB) guided bronchoalveolar lavage (BAL) in patients suspected to have tuberculosis. Methods: This prospective cross-...

The lesser degree of acid-fastness of lepra bacilli as compared with tubercle bacilli appears in a disagreeable manner in the difficulty of demonstrating the organisms of leprosy in paraffinized tissues (1). Faraco (2) showed that by ordinary methods of demonstrating acid-fast organisms the lepra bacilli are often not acid-fast, are not differentiated and may be stained by the counterstain. He ...

BACKGROUND Intestinal tuberculosis can closely mimic Crohn's disease and colon cancer. Presented here is a case of intestinal tuberculosis that closely mimicked both. CASE REPORT A 23 year old Hispanic female presented with several months of weight loss, recurrent fever, and emesis. The patient did not have pulmonary symptoms or radiographic evidence of tuberculosis. Colonoscopy evaluation wi...

objective :cryptosporidiosis is one of the most important parasitic infections in iran which causes diarrhea in humans and animals. the identification of the cryptosporidium species among humans is necessary. this study aims to identify species of cryptosporidium isolated from patients that referred to three hospitals in tehran based on the 18s rrna gene by nested pcr-rflp assay. ‎methods :in t...

introduction: ursolic acid (ua) is a lipophilic triterpenoid compound, found in large amounts in apple peel. anabolic effects of ua on the skeletal muscle and the role of this tissue as a key regulator of systematic aging aroused this question in mind whether ua might amend skeletal muscle performances such as myoglobin expression and also whether it switches skeletal muscle fibers from glycoly...

Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time ( approximately 2 min) to perform. These results suggest that the rapid st...

Background Sputum specimens were received from various out-patient departments of a tertiary care referral hospital to acid-fast staining and mycobacterial culture. Material & Methods A simple, one-step decontamination and concentration method was adopted before subjecting the samples to acid-fast staining and mycobacterial culture. Trisodium phosphate, a cheap, “soft” decontaminant-cum-liquefy...

Background: Nocardia is a Gram-positive and partially acid-fast bacterium. The species are widely distributed in the environment cause severe human infections. Nocardiosis not easily identifiable due to lack of pathognomonic clinical signs. Objectives: present study was designed develop evaluate simple quick method based on loop-mediated isothermal amplification (LAMP) assay for detecting spp i...