نتایج جستجو برای: acid ceramidase

تعداد نتایج: 747667  

Journal: :Journal of lipid research 2013
Luz Camacho Oscar Meca-Cortés José Luis Abad Simón García Nuria Rubio Alba Díaz Toni Celià-Terrassa Francesca Cingolani Raquel Bermudo Pedro L Fernández Jerónimo Blanco Antonio Delgado Josefina Casas Gemma Fabriàs Timothy M Thomson

Acid ceramidase (AC) catalyzes the hydrolysis of ceramide into sphingosine, in turn a substrate of sphingosine kinases that catalyze its conversion into the mitogenic sphingosine-1-phosphate. AC is expressed at high levels in several tumor types and has been proposed as a cancer therapeutic target. Using a model derived from PC-3 prostate cancer cells, the highly tumorigenic, metastatic, and ch...

Journal: :The Journal of biological chemistry 2010
Wei Sun Junfei Jin Ruijuan Xu Wei Hu Zdzislaw M Szulc Jacek Bielawski Lina M Obeid Cungui Mao

Human alkaline ceramidase 2 (ACER2) plays an important role in cellular responses by regulating the hydrolysis of ceramides in cells. Here we report its biochemical characterization, membrane topology, and activity regulation. Recombinant ACER2 was expressed in yeast mutant cells (Deltaypc1Deltaydc1) that lack endogenous ceramidase activity, and microsomes from ACER2-expressiong yeast cells wer...

Journal: :The Biochemical journal 2013
Nagaraju Ramachandra Andreas Conzelmann

Ypc1p (yeast phyto-ceramidase 1) and Ydc1p (yeast dihydroceramidase 1) are alkaline ceramide hydrolases that reside in the ER (endoplasmic reticulum). Ypc1p can catalyse the reverse reaction, i.e. the condensation of non-esterified fatty acids with phytosphingosine or dihydrosphingosine and overexpression of YPC1 or YDC1 can provide enough ceramide synthesis to rescue the viability of cells lac...

2013
Sung-Hyuk Moon Ju-Young Kim Eun-Hwa Song Min-Kyung Shin Yun-Hi Cho Nack-In Kim

BACKGROUND Ceramides are the main lipid component of the stratum corneum and are a structurally heterogeneous and complex group of sphingolipids of which sphingoid bases are the basic structural constituents. Altered levels of sphingoid bases have been reported in skin conditions that involve dryness and barrier disruption, including atopic dermatitis. OBJECTIVE The purpose of this study was ...

2011
Jeremiah M. Draper Zuping Xia Ryan A. Smith Yan Zhuang Wenxue Wang Charles D. Smith

The ceramide/sphingosine-1-phosphate (S1P) rheostat has been hypothesized to play a critical role in regulating tumor cell fate, with elevated levels of ceramide inducing death and elevated levels of S1P leading to survival and proliferation. Ceramidases are key enzymes that control this rheostat by hydrolyzing ceramide to produce sphingosine and may also confer resistance to drugs and radiatio...

Journal: :Journal of virology 2000
J T Jan S Chatterjee D E Griffin

Sindbis virus (SV) causes acute encephalomyelitis by infecting and inducing the death of neurons. Induction of apoptosis occurs during virus entry and involves acid-induced conformational changes in the viral surface glycoproteins and sphingomyelin (SM)-dependent fusion of the virus envelope with the endosomal membrane. We have studied neuroblastoma cells to determine how this entry process tri...

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