prangos ferulacea is a plant found in the mediterranean and middle-east regions used as carminative, anti-flatulent, emollient and antibacterial herb. it is believed that the coumarins are responsible for some of known effects of  prangos . in this research the relaxant effects of p. ferulacea coumrin rich extract as well as osthole as its main prenylated coumarins were investigated on rat ileu...

The microdialysis technique was applied to detect the changes in the activity of acetylcholine (ACh) neurons in the rat brain. The effects of intraperitoneal (i.p.) injection of toluene on the amount of ACh release from the nerve terminals of the brain cholinergic neurons were investigated in freely moving rats. In the striatum, injection of toluene decreased the extracellular concentration of ...

Abstract Background and Aims Renal hemodynamics is impaired since the early stage of cardiometabolic disease. However, in severe obesity, its noninvasive assessment with ultrasound still fails to provide pathophysiologic clinical meaningfulness. The aim study explore relationship between peripheral microcirculation renal obesity. Method We enrolled forty-eight patients affected by obesity an in...

Altered acetylcholine (Ach) homeostasis is associated with loss of viability in flies, developmental defects in mice, and cognitive deficits in human. Here, we assessed the importance of Ach in Caenorhabditis elegans development, focusing on the role of Ach during dauer formation. We found that dauer formation was disturbed in choline acetyltransferase (cha-1) and acetylcholinesterase (ace) mut...

ACSF: Artificial Cerebrospinal Fluid; ACh: Acetylcholine; nAChR: Nicotinic Acetylcholine Receptors; DAergic: Dopaminergic; VTA: Ventral Tegmental Area; SNc: Substantia nigra pars compacta; GABAergic: Gamma-Aminobutyric Acid-Ergic; Glu: Glutamate; Gly: Glycine; Bic: Bicuculline; CNS: Central Nervous System; PD: Parkinson’s Disease; PBS: Phosphate-Buffered Saline; TH: Tyrosine Hydroxylase; H-curr...

Cholinergic signaling, which modulates cell activities via nicotinic and muscarinic acetylcholine receptors (n- and mAChRs) in response to internal or external stimuli, has been demonstrated in mammalian non-neuronal cells that synthesize acetylcholine (ACh). One of the major pathways of excitatory transmission in the enteric nervous system (ENS) is mediated by cholinergic transmission, with th...

Acetylcholine (ACh)-evoked flow from the main excretory duct as well as ACh-induced secretory and resting membrane potentials from cells of the rabbit lacrimal gland were recorded during perfusion with inhibitors. During perfusion with 2,4-dinitrophenol (DNP), ouabain, or ethacrynic acid, ACh-induced flow was 5%, 20%, and 8% of control, respectively; ACh-induced hyperpolarizing secretory potent...

to evaluate the acute effect of lovastatin on diabetic endothelial dysfunction, we examined this effect on the aortic rings of streptozotocin-diabetic rats. the endothelial function was assessed in aortic rings isolated from diabetic rats, 12 weeks after treatment with streptozotocin (45 mg/kg, i.p.). the concentration-response curve to acetylcholine (ach) in the aortic rings precontracted with...

isovanillin and isoacetovanillon are two components found in p. spinosa decne.exboiss extract with no previously reported effect on ileum contractions. spasmolytic effect of isovanillin and isoacetovanillon were examined on response to electrical field stimulation (efs), acetylcholine (ach) and 5-ht in strips of rat ileum. longitudinal ileum strips were set up in an organ bath containing oxygen...

Cyclic GMP relaxes swine tracheal smooth muscle. Relaxation occurs because of decreases in intracellular calcium concentration ([Ca++]i) that are thought to occur through hyperpolarization which inhibits calcium influx. Activation of K+ channels has been suggested as the underlying mechanism for the hyperpolarization. In the present study, the effects of 8-bromoguanosine 3',5'-cyclic monophosph...