Abstract The use of MHC tetramers to identify antigen specific T cells has become widespread among immunologists since the late 90s. Tetramers have so ubiquitous that many companies and research facilities can ship deliver some most commonly used within a matter days. But when it comes uncommon or with antigenic peptides only recently been identified, may be quite challenge find needed tetramer...

Peptide-mediated interactions, in which a short linear motif binds to a globular domain, play major roles in cellular regulation. An accurate structural model of this type of interaction is an excellent starting point for the characterization of the binding specificity of a given peptide-binding domain. A number of different protocols have recently been proposed for the accurate modeling of pep...

Specific binding of the C-peptide of proinsulin was evaluated using a transplantable NEDH rat islet cell tumour predominantly composed of insulin-secreting B-cells. Cultured tumour B-cells exhibited greater than 90% viability assessed by trypan blue exclusion, and retained the ability to form tumours with accompanying hypoglycaemia and hyperinsulinaemia after reimplantation. During binding expe...

A laminin-binding peptide (peptide G), predicted from the cDNA sequence for a 33-kDa protein related to the 67-kDa laminin receptor, specifically inhibits binding of laminin to heparin and sulfatide. Since the peptide binds directly to heparin and inhibits interaction of another heparin-binding protein with the same sulfated ligands, this inhibition is due to direct competition for binding to s...

introduction: it has been shown that some primary human tumors and their metastases, including prostate and breast tumors, over-express gastrin-releasing peptide (grp) receptors. bombesin is a neuropeptide with a high affinity for these grp receptors. the purpose of this study was to prepare and evaluate the characteristics of a new freeze-dried kit, [6-hydrazinopyridine-3-carboxylic acid (hyni...

One of the main functions alpha-2-macroglobulin (A2M) in human blood serum is binding all classes protease . It known that trypsin , after such interaction, possesses modified proteolytic activity Trypsin first hydrolyzes two bonds A2M’s ‘bait region’, and peptide 705 VGFYESDVMGR 715 released from A2M. In this work, specifics A2M-trypsin interaction were used to determine A2M concentration dire...

Peptide-based materials provide a versatile platform for sensing and ion sequestration since peptides are endowed with stimuli-responsive properties. The mechanism of molecular is often based on peptide structural changes (or switching), caused by the binding target molecule. One scope applications selection specific analyte, which may be achieved adjusting structure site. Therefore, exact know...

Complement peptide receptors (nomenclature as agreed by the NC-IUPHAR subcommittee on [113]) are activated endogenous ~75 amino-acid anaphylatoxin polypeptides C3a and C5a, generated upon stimulation of complement cascade. C5a exert their functions through binding to (C3a receptor, receptor 1 2), causing cell recruitment triggering cellular degranulation that contributes local inflammation.