In complement to the JDS4 traditional fish survey (TFS, mainly by electrofishing), a eDNA metabarcoding-based has been implemented within framework of monitoring program organized DNAqua-Net and in collaboration with INTERREG "MEASURES" program. Water samples were collected at 29 sites from source mouth Danube River, 18 tributaries. Two water (mean volume L per sample) each site filtered situ. ...