Smooth muscle myosin light chain kinase (MLCK) is stable in the presence of Ca2+/calmodulin and does not undergo inactivation as reported for skeletal muscle MLCK (Kennelly, P.J., Starovasnik, M.A., Edelman, A.M., and Krebs, E.G. (1990) J. Biol. Chem. 265, 1742-1749). The 61-kDa tryptic fragment of smMLCK-(283-779) with the pseudosubstrate/calmodulin binding sequence deleted undergoes rapid ina...

Thrombomodulin (TM), a membrane proteoglycan on endothelial cells, binds thrombin in a 1:1 complex, accelerates the protein C activation by thrombin, promotes the thrombin inactivation by antithrombin III and inhibits the procoagulant properties of thrombin. The inactivation of single-chain urokinase-type plasminogen activator (scu-PA) by thrombin is accelerated about 70-fold by TM [De Munk, Gr...

This paper presents a magnetostrictive in-pipe impact drive mechanism (IDM). To estimate the output performances of the IDM, a dynamics model was developed based on the magnetostrictive material constitutive model and mechanical model of the IDM. Therefore, an experimental system has been built to test the motion performance of IDM. Simulation and experimental results illustrate that the propos...

There is an immense healthcare challenge and financial pressure due to the COVID-19 pandemic caused by a newly identified human coronavirus, SARS-CoV-2. Effective prevention efforts in healthcare, home, community settings highlight need for rapid, efficient, no-contact SARS-CoV-2 inactivation strategies. Here, we examined photocatalytic virucidal activity of MACOMA™ TiO2 film activated UVA-LED-...

Glibenclamide (GB) is one of the drugs that used in first line for management diabetes mellitus. The aim this work to develop a unique green UPLC analytical procedure analyze GB pharmaceutical products by implementing QbD (Quality Design) approach. 32 full factorial design was applied study effect three levels formic acid (X1; 0.05-0.5%) and temperatures (X2; 25-55oC), their effects on retentio...

The affinity of low density lipoprotein(LDL) to its receptor is very important, because most of LDL-uptake pathway is done by the LDL receptor and the change in size of LDL particle and the modification in its components may affect the LDL affinity for its receptor. In this study, the effects of a powerful lipid-soluble antioxidant “ubiquinol-10” have been investigated on the affinity of LDL to...

Plasmin not only functions as a key enzyme in the fibrinolytic system but also directly inactivates factor VIII and other clotting factors such as factor V. However, the mechanisms of plasmin-catalyzed factor VIII inactivation are poorly understood. In this study, levels of factor VIII activity increased approximately 2-fold within 3 min in the presence of plasmin, and subsequently decreased to...

The affinity of low density lipoprotein(LDL) to its receptor is very important, because most of LDL-uptake pathway is done by the LDL receptor and the change in size of LDL particle and the modification in its components may affect the LDL affinity for its receptor. In this study, the effects of a powerful lipid-soluble antioxidant “ubiquinol-10” have been investigated on the affinity of LDL to...