Seventeen isolates ofMetarhizium anisopliae (Metschnikoff) Sorokin and three isolates of Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) were evaluated for their pathogenicity to the adult pea leafminer, Liriomyza huidobrensis (Blanchard) (Diptera: Agromyzidae), in the laboratory. Flies were contaminated with dry conidia through a velvet material wrapped around the inner s...

Spergualin exhibited a strong antitumor effect against L1210(IMC), a tumor cell line which has been maintained in BALB/c X DBA/2 F1 (hereafter called CD2F1) mice in the Institute of Microbial Chemistry. Mice inoculated i.p. with 10(5) cells of L1210(IMC) survived more than 60 days by daily i.p. administration of spergualin for 9 days at 5 mg/kg/day, which was started 1 day after the tumor inocu...

We have identified an Arabidopsis mutant that displays enhanced disease resistance to the fungus Erysiphe cichoracearum, causal agent of powdery mildew. The edr1 mutant does not constitutively express the pathogenesis-related genes PR-1, BGL2, or PR-5 and thus differs from previously described disease-resistant mutants of Arabidopsis. E. cichoracearum conidia (asexual spores) germinated normall...

A workshop was convened, at which seven enzyme-linked immunosorbent assays (ELISAs) were compared with virus isolation for the detection of viraemia in serial blood samples collected from six pigs at up to fourteen days after inoculation with classical swine fever virus. All ELISAs were of the double antibody sandwich type, using monoclonal and/or polyclonal antibodies to detect a variety of vi...

activities of thirty bacterial strains obtained from plants rhizosphere in different areas against phytophthora drechsleri were assessed through in vitro and greenhouse experiments. dual culture and extracellular metabolites tests were used in in vitro assays. the bacterial strains inhibited growth of p. drechsleri, the highest inhibition zones was belonged to t17-4 strain with 61.904% in dual ...

Transformation of Japanese encephalitis antibody from IgM to IgG in the sera of the experimental infected chicks with Japanese encephalitis virus and transmission of IgM or IgG from hen to chicks were examined by the gel filtration on Sephadex G-200 column. The following results were obtained. 1. Titer of hemoagglutination inhibiting antibody rose on seven days after inoculation of mouse brain ...

Studies were conducted to develop mild/avirulent strain of PRSV-W using nitrous acid, sodium azide and UV-radiation for the mutagenic treatments. Among the three mutagens’ sodium azide at 3%, 4%, and 5%, and nitrous acid at 0.1M and 0.125M were proved to be effective in producing mild/avirulent strains of PRSV-W, while UV-radiation was proved to be ineffective. Evaluation on the effectivity of ...

Fungicides used to control Fusarium head blight (FHB) are commonly applied at the wheat growth stage considered be most susceptible, i.e., anthesis. We compared efficacy of fungicide groups that were following two strategies: (i) pre-defined stages, from first half heading end flowering (experiment 1, in 2013 2015), or (ii) based on timing infection by F. graminearum, specifically 10, 7, 4, 1 d...

The virucidal effect of pH and salinity on Penaeus monodon baculovirus (MBV) was investigated by water borne inoculated methods exposing PL12 Penaeus semisulcatus for 10 hours and observing for 10 days at temperature 28- 30°C. For this purpose, one thousand virus free PL12 of P. semisulcatus were divided into five groups and each group placed in a 10 L plastic basin containing chlorine-treated ...

Intracerebral inoculation of field-isolates as well as established strains of equine herpesvirus-1 (EHV-1) in suckling mice results in viral replication in neurons and glial cells and induces encephalitis. By intraperitoneal (i.p.) inoculation, no histological lesion was observed in the central nervous system (CNS) in suckling mice with the EHV-1 HH1 strain (HH1), whereas a neuroadapted variant...