Pichia pastoris is a widely used host for the production of heterologous proteins. In this case, high cell densities are needed and oxygen is a major limiting factor. The increased air pressure could be used to improve the oxygen solubility in the medium and to reach the high oxygen demand of methanol metabolism. In this study, two P. pastoris strains producing two different recombinant protein...

The replacement in the diet of refined carbohydrate and fat with fibre and protein has been shown to promote satiety and improve glucose and insulin profiles. It is less clear whether the macronutrient composition of individual foods such as snacks have any meaningful impact on metabolic parameters and satiety. We examined if the consumption of higher protein higher fibre snack bars would resul...

The use of porous materials to store natural gas in vehicles requires large amounts of methane per unit of volume. Here we report the synthesis, crystal structure and methane adsorption properties of two new aluminum metal-organic frameworks, MOF-519 and MOF-520. Both materials exhibit permanent porosity and high methane volumetric storage capacity: MOF-519 has a volumetric capacity of 200 and ...

Materials and Methods: Seventy-six patients who received two or four interforaminal implants were assigned to one of three different bar designs and subsequently to different prosthesis supporting systems. Forty-nine patients received implants and a mucosa-supported implantretained overdenture (OD) with an ovoid bar (two implants; design 1) or multiple ovoid bars (four implants; design 2). Twen...

In this paper we classify all block equalities and all nontrivial block inclusions for spin blocks of the double covers of the symmetric groups at different odd primes. More generally, we describe for an odd integer s > 1 explicitly when an s̄-block of bar partitions is contained in a t̄-block of bar partitions, for t > 1 an odd integer or t = 4. The question of block equality leads to the study ...

An aquaculture system can be a potentially significant source of antibacterial compounds and ammonia in an aquatic environment. In this study, the removal of total ammonia nitrogen and florfenicol antibiotic from synthetic aqueous wastewater was assessed by applying a commercial TFC (thin film composite) polyamide nanofilter. The effects of pH (6.5-8.5), pressure (4-10 bar), concentration of to...

Oligonucleotides targeting M5 muscarinic receptor mRNA were infused for 6 d into the ventral tegmental area of freely behaving rats trained to bar-press for lateral hypothalamic stimulation. The bar-pressing rate was determined at a range of frequencies each day to evaluate the effects of infusions on reward. M5 antisense oligonucleotide (oligo) infusions increased the frequency required for ba...

we have shown that buspirone, a partial agonist of 5-hydroxytryptamine 1a (5-ht1a) receptors, improves motor dysfunctions induced by 6-hydroxydopamine (6-ohda) and haloperidol in rats. the present work extends these findings by investigating the role of 5-ht1a receptors on catalepsy-like immobilization in rats, a model of parkinson’s disease. catalepsy was induced by unilateral infusion of 6-oh...

Statistics of mitochondrial association with spindle poles in wild type and mto1∆ (Figures 1D and 2B) Out of the 35 imaged wild-type cells, 18 cells showed constant mitochondrial association (red bar in Figure 1D), 6 showed at least one spindle pole free from mitochondria for at least 5 minutes (green bar in Figure 1D), 6 displayed temporary mitochondrial detachment (not included in the compari...