background: progress in the field of biology and biochemistry has led to the discovery of numerous bioactive peptides and proteins in the last few decades. delivery of therapeutic proteins/peptides has received a considerable amount of attention in recent years. methods: in this study, a two-step desolvation method was used to produce biodegradable hydrophilic gelatin nanoparticles (gnp) as a d...

three new rimantadine schiff bases (rsbs) were prepared, and then the interaction of rsbs with bovine serum albumin (bsa) was investigated using fluorescence, synchronous fluorescence, uv-vis absorption spectroscopy under physiological conditions. the results showed that the three rsbs effectively quenched the intrinsic fluorescence of bsa via static quenching. binding constant (ka), number of ...

INTRODUCTION It has been demonstrated that organic content of the root canals can influence the antimicrobial capability of chemical irrigants. The aim of this study was to evaluate the effect of bovine serum albumin (BSA), as an organic material, on the antimicrobial activity of several intracanal irrigants. MATERIALS AND METHODS Bactericidal activity of Ethylenediaminetetraacetic acid (EDTA...

Effects of maternal dietary polyunsaturated fatty acids (PUFA) on the spleen fatty acid composition and BSA-induced wing web swellings were investigated in broilers. One hundred twenty broiler breeder hens 26 wk of age were randomly assigned to diets containing mainly wheat, corn, soy meal, barley, oat and 5% (wt/wt) added sunflower oil, fish oil, or a mix of sunflower and fish oils (1:1). Afte...

Although the importance of estradiol-17beta (E(2)) in many physiological processes has been reported, to date no researchers have investigated the effects of E(2) on embryonic stem (ES) cell proliferation. Therefore, in the present study, we have examined the effect of E(2) on the DNA synthesis of murine ES (ES-E14TG2a) cells and its related signaling pathways. The results of this study show th...

The reaction between reducing sugars and protein free amines, known as the Maillar reaction results in the formation of advanced glycation endproducts (AGEs). AGE modification changes the structure of proteins to amyloid cross-beta structure. These protein structures can activate receptors known as RAGE on glial cells (microglia and astrocytes), and induce the expression of inducible nitric oxi...

Heat induced denaturation of bovine serum albumin (BSA) in dimethylsulfoxide (DMSO) containing solutions in the presence of potassium iodide has been studied using UV/vis spectroscopy method. It has been shown that the thermal stability of protein depends on competitive interactions taking place in the solution. At low DMSO concentrations (5 vol. %) BSA is more stable than at higher concentrati...

In this research, the optimum conditions for the separation of polyphenols from apple juice (AJ) and the separation of BSA from a BSA/water solution were found by looking at the enrichment ratio (ER) and recovery. A mixture of the AJ and BSA was also studied to see how the different conditions affect separation of either compound. For AJ only, maximum ER was achieved at a feed concentration of ...

The cytochrome P450 (P450)-dependent conversion of phenytoin (PHT) to p-hydroxy phenytoin (pHPPH), and tolbutamide (TLB) to 4-hydroxy tolbutamide (hydroxy-TLB), in human liver microsomes was studied in the presence of increasing concentrations (0-4%) of bovine serum albumin (BSA). Therefore, the free fraction (f(u)) of PHT and TLB varied. Whereas the f(u) of PHT (5 microM) decreased, an increas...

Electron microscopy was used to evaluate the function and formation of dense core lysosomes. Lysosomes were preloaded with bovine serum albumin (BSA)-gold conjugates by fluid phase endocytosis using a pulse-chase protocol. The gold particles present in dense core lysosomes and late endosomes were flocculated, consistent with proteolytic degradation of the BSA. A second pulse of BSA-gold also ac...