نتایج جستجو برای: 5 شلایرماخر2

تعداد نتایج: 1215766  

2015
Sabrina M Huber Pieter van Delft Lee Mendil Martin Bachman Katherine Smollett Finn Werner Eric A Miska Shankar Balasubramanian

RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m(5)C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm(5)C) and 5-formylcy...

Journal: :Nucleic acids research 1997
R K Bruick M Koppitz G F Joyce L E Orgel

A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino- 5'-deoxythymidine in the presence of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligati...

Journal: :Nucleic acids research 1993
K Yazdanbakhsh P Fraser D Kioussis M Vidal F Grosveld M Lindenbaum

We have carried out a structural and functional analysis on the human NF-L (H-NF-L) gene. It contains a methylation-free island, spanning the 5' flanking sequences and the first exon and a number of neuronal-specific DNase I hypersensitive sites have been identified in the upstream region as well as within the body of the gene. Analysis in cell lines and transgenic mice using a combination of t...

Journal: :Acta biochimica Polonica 2013
Pavla Moricová Vladan Ondřej Božena Navrátilová Lenka Luhová

Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation...

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