Whole genome duplication (WGD) and tandem duplication (TD) are both important modes of gene expansion. However, how WGD influences tandemly duplicated genes is not well studied. We used Brassica rapa, which has undergone an additional genome triplication (WGT) and shares a common ancestor with Arabidopsis thaliana, Arabidopsis lyrata, and Thellungiella parvula, to investigate the impact of geno...

The objective of present study was to formulate directly compressible orodispersible tablets of quetiapine fumarate by sublimation method with a view to enhance patient compliance. A full 32 factorial design was used to investigate the effect of two variables viz., concentration of Indion 414 and camphor. Indion 414 (3-5 % w/w) was used as superdisintegrant and camphor (5-15 % w/w) as subliming...

Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points in amylopectin. Enhancing its thermostability is required for its industrial application. In this study, rational protein design was used to improve the thermostability of PulB from Bacillus naganoensis (AB231790.1), which has strong enzymatic properties. Three positive single-site mutants (PulB-D328H,...

Joaquín Álvarez BarrientosMaquetista y artillero. León Gil de Palacio (1778-1849), entre ciudad patrimonioZaragoza: Prensas la Universidad Zaragoza, 2022. 414 pp. Tapa blanda. Idioma: español. 28 €ISBN: 978-84-1340-406-6

We read carefully the very interesting article by Jones et al. (1), which appears in the latest issue of the Journal. To our surprise, we realized that, during the determination of opiates in blood, although the described derivatization procedure involves BSTFA, the authors use, for their qualitative and quantitative analysis, mass fragments that correspond to their pentafluoroderivatives and t...