Inorganic arsenic (iAs) is a known human carcinogen at high exposures, increasing the incidences of urinary bladder, skin, and lung cancers. In most mammalian species, ingested iAs is excreted mainly through urine primarily as dimethylarsinic acid (DMA(V)). In wild-type (WT) mice, iAs, DMA(V), and dimethylarsinous acid (DMA(III)) exposures induce formation of intramitochondrial urothelial inclu...

The aimed of this study was to evaluate curcumin as anticancer agent. The apoptogenic and Cytotoxic activity of curcumin were investigated against two human leukemic cell lines-U937 and Molt4. Cytotoxic activity was performed by using [3-[4, 5-dimethylthiazol-2yl] 2, 5 diphenyltetrazolium bromide] MTT assay. Also 4, 6-diamidino-2-phenylindole di-hydrochloride [DAPI] staining was used as a DNA-b...

OBJECTIVE Expression of Notch1 gene in lung cancer A549 cells was reduced using small interfering RNA (small interfering RNA, siRNA) and the effect of Notch1 gene on proliferation and chemo sensitivity of lung cancer A549 cells was studied. MATERIALS AND METHODS The Notch1 siRNA was transfected into A549 cells by liposome to inhibit the expressions of Notch1 gene in A549 cells. Real-time poly...

The aim of the present study was to examine the effect of fraxetin on proliferation and apoptosis in the MCF-7 breast cancer cell line. Cell proliferation was measused using an MTT assay and 4',6-diamidino-2-phenylindole (DAPI) staining was used to determine shrinkage and condensation. RT-PCR was used to examine the expression of factor-associated suicide (Fas) and Fas ligand (FasL) mRNA, and w...

amorphophallus campanulatus (roxb.) blume belonging to the family of araceae, is a perennial herb commonly known as elephant foot yam. its tuber has been traditionally used for the treatment of liver diseases, abdominal tumors, piles. the aim of the present study was to evaluate the dose-dependent cytotoxic and apoptosis inducing effects of the sub fractions of amorphophallus campanulatus tuber...

methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

Platinum based combined chemotherapy have been proved to be the most effective drugs for ovarian cancer treatment, but it is difficult treat cisplatin resistant cancer. Carbobenzoxy-L-leucy-L-Leucyl-L-Leucinal (MG132) a reversible tripeptide aldehyde proteasome inhibitor, purpose of this study was observe effect MG132 on SKOV3 cell and OVCAR-3 expression autophagy apoptosis related factors. The...