Polyoma virus-transformed baby hamster kidney (pyBHK) cells were cultured in medium containing [32P]orthophosphate and 10% (vol/vol) fetal bovine serum. A 32P-labeled protein with an apparent molecular mass of 97 kDa was immunoprecipitated from cell lysates with antiserum to ADP-ribosylated elongation factor 2 (EF-2). The 32P labeling of the protein was enhanced by culturing cells in medium con...

Potato spindle tuber viroid (PSTV) was radioactively labeled during its synthesis in PSTV-infected tomato plants whose roots had been placed in nutrient solution containing [32P]orthophosphate. The 32P was incorporated into PSTV when the radioactive label was introduced into infected plants ‘2 weeks after inoculation, when systemic symptoms of PSTV started to appear. Synthesis of 32P-labeled PS...

Dear Editor, I read with great interest the article authored by Dr. Leelavathi Basava et al. titled ‘‘Falope Ring or Modified Pomeroy’s Technique for Concurrent Tubal Sterilization ‘‘published in your (September–October 2016) 66 (S!): S198–S201. I would like to congratulate the authors for an excellent study in the search of a method for female sterilization technique, which is quicker and easi...

In intracavitary radiotherapy (ICRT) for cancer cervix, applicator geometry has the potential to impact the doses to organs at risk (OARs) and the coverage of the target volume. At our centre two Varian made Fletcher-style applicator sets, namely defined (fixed) geometry and flexible geometry, are used for ICRT. In the present work, the two types of applicators were compared dosimetrically as p...

PURPOSE To investigate the dosimetric results and first clinical experiences with a new designed balloon applicator with adjustable catheters for endoluminal brachytherapy for patients with locally advanced rectal cancer not undergoing surgery. MATERIAL AND METHODS The applicator consists of an inflatable rectal balloon with six attached Foley catheters used as guidance for the inserted brach...

BACKGROUND Optimal applicator insertion is a precondition for the success of cervix cancer brachytherapy (BT). We aimed to assess feasibility and efficacy of MRI-assisted pre-planning, based on applicator insertion in para-cervical anaesthesia (PCA). PATIENTS AND METHODS Five days prior to BT, the pre-planning procedure was performed in 18 cervix cancer patients: tandem-ring applicator was in...

Background: Surgical site infection (SSI) remains a concern in shoulder surgery, especially during arthroplasty. Whilemany studies have explored the characteristics and efficacy of different sterilizing solutions, no study has evaluated themethod of application. The purpose of this study was to compare two popular pre-surgical preparatory applications (two4 x 4 cm gauze sponges and applicator s...