نتایج جستجو برای: 30 expression vector

تعداد نتایج: 1528184  

Journal: :iranian journal of pharmaceutical research 0
mohammad hossein morowvat department of pharmaceutical biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences, valiollah babaeipour biochemical engineering group, biotechnology research center, malek-ashtar university of technology hamid rajabi-memari center of biotechnology and life sciences, shahid chamran university of ahvaz hossein vahidi department of pharmaceutical biotechnology, faculty of pharmacy, shahid beheshti university of medical sciences nader maghsoudi neuroscience research center, shahid beheshti university of medical science

human interferon β (inf-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. in this study an expression vector was designed and constructed for expression of human inf-β-1b either in shake flasks and bench top bioreactor. the designed vector was constructed based upon pet-25b(+) with t7 promoter. recombinant human beta interferon ...

Journal: :jundishapur journal of microbiology 0
reza taherkhani health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran fatemeh farshadpour health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran manoochehr makvandi health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical science, ahvaz, ir iran. tel: +98-9166181683, fax: +98-6113738313 ali reza samarbafzadeh health research institute, infectious and tropical disease research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

Amiri F, Habibi Roudkenar M, Jalili M, Mehrabi Habibabadi H, Moslemi E,

Background: Mesenchymal stem cells (MSCs) are ideal cells for cell and gene therapy. However, the low survival of MSCs after transplantation has limited their applications. We aimed to evaluate the expression of cytoprotective genes including NQO1, TXNRD1, HO-1, GCLC following the over expression of Nrf2 in umbilical cord-derived MSCs (UC-MSCs). Methods: 3-5 passages of UC-MSCs were cultured. ...

B TABARRAI, MH SHAH-HOSSEINY, MR AKBARI, V RECHINSKY,

Knowing the nucleotide sequence of the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of V. cholerae. The resulting amplification product was cloned in a common pUC18 vector. Subsequently, a part of this operon encoding the cholera toxin Bsubunit (CTB) was reamplified and cloned between the BamH1 and EcoR1 sites of the same ...

SFL1 and SFl2 (SETH Four Like) genes are two members of SETH4 gene family in Arabidopsis thaliana expressed in saprophytic tissues. In this study, expression of SFL1 and SFL2 genes were studied using Gateway Cloning Technology. Primers were designed for PCR amplification of promoter region of SFL1 (900 bp) and SFL2 (930 bp) genes having attB1 recombination sites using Kod Hi Fi DNA polymerase e...

Journal: :journal of sciences islamic republic of iran 0

sfl1 and sfl2 (seth four like) genes are two members of seth4 gene family in arabidopsis thaliana expressed in saprophytic tissues. in this study, expression of sfl1 and sfl2 genes were studied using gateway cloning technology. primers were designed for pcr amplification of promoter region of sfl1 (900 bp) and sfl2 (930 bp) genes having attb1 recombination sites using kod hi fi dna polymerase e...

Toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. Toxoplasma gondii-derived heat shock protein 70 (Hsp70) may play an important role in the virulence of Toxoplasma gondii (T. gondii). In the present study, T. gondii Hsp70 was amplified by polymerase chain reaction (PCR) from the DNA of the T. gondiitachyzoite RH strain through the use o...

Background: Corynebacterium glutamicum (C. glutamicum) is a potential host for the secretory production of the heterologous proteins. However, to this date few secretion-type gene expression systems in C. glutamicum have been developed, which limit applications of C. glutamicum in a secretory production of the heterologous proteins.Objectives: In this stu...

Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks and bench top bioreactor. The designed vector was constructed based upon pET-25b(+) with T7 promoter. Recombinant human beta interferon ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد 1376

in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...

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