ackground: Knowing the human platelet antigens (HPA) and genes frequency in different populations is important not only for population studies but also for clinical transfusion practice. HPA genes frequency in Iran is not evaluated, so we investigated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 patie...

A variety of methods have been developed for cloning PCR products, including blunt-end cloning (1), restriction cut back (2), ligation-independent cloning (3), uracil DNA–glycosylase (UDG) treatment of uracil-containing deoxyoligonucleotide primers (4,5) and TA cloning (6–8). Blunt-end cloning of PCR products often requires treatment of PCR products to polish the ends (9). Even with treatments,...

Gestational diabetes is a glucose intolerance level first identified during pregnancy. This condition caused by hormone secretion that hinders insulin action from the placenta which resistance will cause hyperglycemia. increase oxidative stress, and in consequence increasing inflammation cell death can hinder fetal growth development. Salacca zalacca peel food waste contains antioxidants anti-i...

An efficient, PCR based method for the selective amplification of DNA target sequences that differs by a single base pair is described. The method utilises the high affinity and specificity of PNA for their complementary nucleic acids and that PNA cannot function as primers for DNA polymerases.

The paper describes a developed comprehensive system for molecular-genetic identification of Yersinia pestis strains according to their appurtenance certain subspecies, biovars, phylo-geographic populations, using realtime PCR (RT-PCR), allele-specific RT-PCR, and multiplex with hybridization fluorescent registration results on solid substrate. Application this makes it possible establish the Y...