BackgroundThe prevalence of atopic dermatitis (AD) and allergic or irritant contact has been increasing significantly in the general population. Interleukin- 4 (IL-4), interleukin-6 (IL-6), immunoglobulin E (IgE) play a key role pathogenesis AD. Physalis angulata (PA) leaves reportedly have anti-inflammatory effects by impeding IL-4, IL-6, IgE. This study aimed to evaluate effect PA leaf extrac...

Fc receptors for IgE (Fc epsilon R) on human peripheral blood lymphocytes and monocytes and cultured lymphoblastoid and macrophage-like cell lines were compared with respect to: 1) binding affinity for radiolabeled IgE, 2) inhibition of IgE-specific rosette formation and inhibition of binding of radiolabeled IgE by an antiserum raised against Fc epsilon R isolated from a lymphoblastoid cell lin...

An immunotherapeutic vaccine for allergy was produced by designing IgE-based synthetic peptide immunogens and selecting them for functional immunogenicity. The vaccine targets the binding site on IgE for the high affinity receptor Fc epsilon RI, by active immunization. The peptide target site on IgE heavy chain was selected from among the amino acid sequences for the C epsilon 2, C epsilon 3, a...

Monitoring free serum IgE in severe asthma patients treated with omalizumab. 2 Omalizumab is effective in allergic and nonallergic patients with nasal polyps and asthma. 2 Effects of adding omalizumab, an anti-immunoglobulin E antibody, on airway wall thickening in asthma. 3 A genome-wide association study of plasma total IgE concentrations in the Framingham Heart Study. 4 Reduction of total Ig...

IL-3 is a potent priming cytokine for human basophils, inducing an increase of mediator release after stimulation. The mechanism of IL-3 priming of the basophil response to FcepsilonRI aggregating stimuli remains unknown. We explored the regulation of several elements of IgE-mediated signaling by a short priming with IL-3. Early signaling events such as phosphorylation of Syk, Shc, linker for a...

It has been shown that preferential production of IgE antibody may be obtained in certain strains of mice if the adjuvant is Al(OH)3 (1). A high and persistent titer of IgE antibody can thus be obtained using minute doses of antigen and even booster responses can be produced (2). If mice are primed with microgram amounts of dinitrophenylated keyhole limpet hemocyanin (DNPKLH) ~ with AI(OH)3 as ...

Resistance to Schistosoma mansoni infection has been correlated with IgE responses to the adult worm. Molecular targets of this response are gaining interest as markers of immunity and as indicators of allergenic properties. Few protein families contain IgE antigens (allergens) and one of the most highly represented are the tropomyosins. Alternative splicing generates numerous tropomyosin isofo...

As reported previously, and confirmed here in 26 donors, the serum IgE level (2.6-5,500 ng/ml) correlates well (rs = 0.95, P less than 0.001) with the in vivo number of IgE molecules/basophil (6,000-600,000). The total number of IgE receptors/basophil was monitored by incubating them with an IgE-rich serum (15 microgram/ml), quantitatively stripping IgE from the cells at pH 3.7, and measuring e...

Cypress pollen represents the primary cause of respiratory allergies in Mediterranean areas. Patients allergic to Cupressus sempervirens pollen (Cups) (CPA) can be discriminated on the basis of the immunoglobulin E (IgE) binding to a basic 14 kDa protein (BP14) or to high-molecular-weight (HMW) glycoproteins only. Specific IgE repertoires of two differentially exposed CPA cohorts, French and It...