Silver nitrate staining of decalcified bone sections is known to reveal osteocyte canaliculi and cement lines. Nucleolar Organising Regions (NOR) are part of the nucleolus, containing argyrophilic proteins (nucleoclin/C23, nucleophosmin/B23) that can be identified by silver staining at low pH. The aim of this study was to clarify the mechanism explaining why AgNOR staining also reveals osteocyt...

BACKGROUND Argyrophilic nucleolar organizer regions (AgNORs) and MIB-1 as proliferating activities have been applied separately to assess the malignant potential of cancer cells. We conducted staining of AgNORs and MIB-1 in 42 surgically-resected invasive breast carcinomas. MATERIALS AND METHODS Paraffin-embedded sections were used for double staining and the mean AgNOR counts in 100 MIB-1-po...

BACKGROUND The amount of argyrophilic nucleolar organiser regions (AgNORs) represents a cell kinetics parameter used in tumour pathology for prognostic purposes. AgNOR expression is directly related to the rate of ribosome biogenesis, which has been recently shown to be controlled also by the tumour suppressor proteins pRb and p53. AIMS To ascertain the relative prognostic value of AgNORs and...

The prognostic significance of AgNOR proteins in stage II-III rectal cancers treated with chemoradiotherapy was evaluated. Silver staining was applied to the 3μm sections of parafin blocked tissues from 30 rectal cancer patients who received 5-FU based chemoradiotherapy from May 2003 to June 2006. The microscopic displays of the cells were transferred into the computer via a video camera. AgNOR...

Preinvasive & invasive cervical lesions are a major public health concern, though it is a preventable problem to a great extent. At present few reports have been published concerning the variability of proliferative activity in cervical biopsy in women with cervical neoplastic damage. Again though the association between HPV & neoplastic cervical lesions is well known, only a few studies have b...

BACKGROUND The health risks of crack cocaine smoking on the oral mucosa has not been widely researched and documented. OBJECTIVE The purpose of this study was to analyze the proliferative activity of oral epithelial cells exposed to crack cocaine smoke using silver nucleolar organizer region (AgNOR) staining. METHODS Oral smears were collected from clinically normal-appearing buccal mucosa ...

Objectives The aim of this study is finding a practical and easy way to differentiate, between two malignant tumors Adenoid Cystic Carcinoma (ACC) and Polymorphous Low-grade Adeno Carcinoma (PLGA) histologically. In this regard, We proposed silver nitrate staining (AgNOR). Methods In this cross-sectional study, 30 parrafin-embedded blocks of ACC and 9 paraffin-embedded b...

U bladder cancer is a common disease. In Iraq, it represents 12.3% of cancer cases among men, and 4.9% of cancer cases among women.1 Cytological examination of urinary specimens is a relatively simple diagnostic procedure that can aid in the diagnosis of disease anywhere in the urinary tract; and nowadays, examination of urine cytology is regarded as part of the routine investigation of patient...