Results: Serum samples became PCR-positive before semen samples. During the first 6 days after inoculation, serum was PRRSV-positive in 36 of 40 boars, and semen was PRRSV-positive in four of 40 boars. Median time to detection was 36 and 48 hours for nested PCR and Taqman PCR, respectively. Results were inconsistent when a positive semen sample was pooled with negative semen. Elevated rectal te...