DNA methods have resulted in improved renal allograft survival rates in cadaveric renal transplantation. This paper describes the impact of DNA typing by PCRSSP on a living related renal transplant (LRRT) programme. It evaluates error rates in serology, acute rejections, graft function and survival rates between the two typing methods. Serological typing was done on CTS 120 antisera Class 1 and...

BACKGROUND Changing perspectives on the natural history of celiac disease (CD), new serology and genetic tests, and amended histological criteria for diagnosis cast doubt on past prevalence estimates for CD. We set out to establish a more accurate prevalence estimate for CD using a novel serogenetic approach. METHODS The human leukocyte antigen (HLA)-DQ genotype was determined in 356 patients...

background and objective: opportunistic infections are the leading cause of death among patients subjected to the human immunodeficiency virus (hiv) infection and acquired immune deficiency syndrome (aids). the aim of this study was to compare the seroprevalence of cytomegalovirus (cmv)  and toxoplasmosis infection in newly diagnosed hiv infected patients with healthy controls and it’s correlat...

BACKGROUND Autoimmune serologies are often obtained in the initial evaluation of uncharacterized interstitial lung disease (ILD). Whether this practice is helpful in delineating connective-tissue disease related ILD (CTD-ILD) is not well known. We assessed the frequency of incident CTD-ILD as detected by autoimmune serology testing and presenting clinical signs and symptoms. METHODS Consecuti...

background : the ifa test is one of the most usual methods for detecting anti- toxoplasma antibod­ies, although it has not any unique standardization. it seems that the microscopic judg­ment of results is an important confounder in ifa test. therefore, we conducted the present study to clarify the role of microscopic observer, and other confounders on the test. methods: eighty sera were collect...

Case presentation: Girl, 4 years old, healthy. Admitted with right hemiplegia, central facial paralysis and aphasia over 15 days. Vaccines for triple viral influenza given in the previous month. No recent infection. Magnetic Resonance Imaging (MRI) angiography showed hypersignal on T2/FLAIR nucleus-thalamo-capsular region, corona radiata external capsule left, without vasculopathy. Negative ser...

A qPCR array was designed to facilitate forensic serological identification of unknown stains and fluids. In our approach, the flow-through of a DNA purification column is further purified for total RNA. Therefore, the DNA preparation is fully available for forensic DNA analysis whereas the RNA fraction is processed for serological examination by mRNA analysis. The RNA fraction is first subject...

Although automation was introduced into biochemistry in 1957 (Skeggs, 1957), it was not until 1963 that McNeil, Helrick, and Ferrari (1963) first described a mechanized technique forABO grouping. This was followed a few months later by a method employing the AutoAnalyzer continuous flow principle by which up to 40 samples per hour could be both ABO and Rh (D) grouped (Sturgeon, Cedergren, and M...

chemical foundation of these disciplines. The author avoids the principal pedagogic sin of talking, in an equal tone of voice, of subjects of widely varying importance. This is clearly one man's account, but it is balanced, reasonable, and so lucid that one can read it with pleasure. It is a pity that the production of the book is not of as high a standard as its contents. In the review copy a ...